dbacp00813
General Description
Peptide name : A5
Source/Organism : Cyclin B (285-296)
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : KAQIRAMECNIL
Peptide length: 12
C-terminal modification: Linear
N-terminal modification : Free
Non-natural peptide information: None
Activity Information
Assay type : MTT/MTS assay
Assay time : 24h
Activity : Not found
Cell line : HCT-116
Cancer type : Colon cancer
Other activity : Not found
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 1389.6866 Dalton
Aliphatic index : 1.141
Instability index : 98.45
Hydrophobicity (GRAVY) : 0.1583
Isoelectric point : 8.2222
Charge (pH 7) : 0.752
Aromaticity : 0
Molar extinction coefficient (cysteine, cystine): (0, 0)
Hydrophobic/hydrophilic ratio : 1.4
hydrophobic moment : 0.0456
Missing amino acid : W,H,T,P,F,S,D,Y,V,G
Most occurring amino acid : A
Most occurring amino acid frequency : 2
Least occurring amino acid : K
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0.5, 0.0, 0.2)
SMILES Notation: CC[C@H](C)[C@H](NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CS)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCSC)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCCCN)[C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | HHHHHHHHHHHH |
| Chou-Fasman (CF) | HHHHHHHHCCCC |
| Neural Network (NN) | CCHHHHHHHHHH |
| Joint/Consensus | HHHHHHHHHHHH |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
Reference
1 : Lou H, et al. A novel peptide from alpha5 helix of Asterina pectinifera cyclin B conjugated to HIV-Tat(49-57) with cytotoxic and apoptotic effects against human cancer cells. Bioorg Med Chem Lett. 2008; 18:4633-7. doi: 10.1016/j.bmcl.2008.07.017
Literature
Paper title : A novel peptide from alpha5 helix of Asterina pectinifera cyclin B conjugated to HIV-Tat(49-57) with cytotoxic and apoptotic effects against human cancer cells.
Doi : https://doi.org/10.1016/j.bmcl.2008.07.017
Abstract : A series of novel peptides from various motifs of Asterina pectinifera cyclin B and their derivatives conjugated to HIV-Tat(49-57) were designed and synthesized. Their bioactivities on two human cancer cell lines were determined. Among them, Tat-a5 (KAQIRAMECNILGRKKRRQRRR) exhibited significant cytotoxic effects on cancer cell lines EC-9706 and HCT-116. Tat-a5 could arrest cancer cells at G(2)/M phase and make them apoptotic. Our results suggested that Tat-a5 could be a novel leading peptide with anticancer activity.