Peptide name : Alloferon 2

Source/Organism : Blow fly

Linear/Cyclic : Not found

Chirality : Not found

Peptide length: 12

C-terminal modification: Not found

N-terminal modification : Not found

Non-natural peptide information: None

Assay type : Cytotoxicity assay, Antitumor activity assay

Assay time : 28h

Activity : Not found

Cell line : K562

Cancer type : Not found

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 1128.1582 Dalton

Aliphatic index : 0.483

Instability index : -16.758

Hydrophobicity (GRAVY) : -0.625

Isoelectric point : 7.0224

Charge (pH 7) : 0.0216

Aromaticity : 0

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 1.4

hydrophobic moment : -0.765

Missing amino acid : C,R,W,T,P,M,I,E,K,F,D,Y,L,N,A

Most occurring amino acid : G

Most occurring amino acid frequency : 5

Least occurring amino acid : S

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0, 0.5, 0.1)

SMILES Notation: CC(C)[C@H](NC(=O)CN)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](Cc1c[nH]cn1)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)NCC(=O)N[C@H](C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)NCC(=O)O)C(C)C

1 : Chernysh S, et al. Antiviral and antitumor peptides from insects. Proc Natl Acad Sci U S A. 2002; 99:12628-32. doi: 10.1073/pnas.192301899

Paper title : Antiviral and antitumor peptides from insects.

Doi : https://doi.org/10.1073/pnas.192301899

Abstract : Insects can rapidly clear microbial infections by producing a variety of immune-induced molecules including antibacterial and/or antifungal peptides/polypeptides. In this report, we present the isolation, structural characterization, and biological properties of two variants of a group of bioactive, slightly cationic peptides, referred to as alloferons. Two peptides were isolated from the blood of an experimentally infected insect, the blow fly Calliphora vicina (Diptera), with the following amino acid sequences: HGVSGHGQHGVHG (alloferon 1) and GVSGHGQHGVHG (alloferon 2). Although these peptides have no clear homologies with known immune response modifiers, protein database searches established some structural similarities with proteins containing amino acid stretches similar to alloferon. In vitro experiments reveal that the synthetic version of alloferon has stimulatory activities on natural killer lymphocytes, whereas in vivo trials indicate induction of IFN production in mice after treatments with synthetic alloferon. Additional in vivo experiments in mice indicate that alloferon has antiviral and antitumoral capabilities. Taken together, these results suggest that this peptide, which has immunomodulatory properties, may have therapeutic capacities. The fact that insects may produce cytokine-like materials modulating basic mechanisms for human immunity suggests a source of anti-infection and antitumoral biopharmaceuticals.