dbacp01866
General Description
Peptide name : Bombinin H2
Source/Organism : Yellow-bellied toad, Europe
Linear/Cyclic : Not found
Chirality : D
Sequence Information
Sequence : LIGPVLGLVGSALGGLLKKI
Peptide length: 20
C-terminal modification: Not found
N-terminal modification : Amidation
Non-natural peptide information: None
Activity Information
Assay type : Not specified
Assay time : 24h
Activity : Not found
Cell line : Not found
Cancer type : Not found
Other activity : Anti-bacterial activity; Hemolytic activity
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 1918.4096 Dalton
Aliphatic index : 1.9
Instability index : 12.565
Hydrophobicity (GRAVY) : 1.49
Isoelectric point : 10.002
Charge (pH 7) : 1.7581
Aromaticity : 0
Molar extinction coefficient (cysteine, cystine): (0, 0)
Hydrophobic/hydrophilic ratio : 5.66666666
hydrophobic moment : 0.0889
Missing amino acid : C,R,W,H,Q,T,M,E,F,D,Y,N
Most occurring amino acid : L
Most occurring amino acid frequency : 6
Least occurring amino acid : P
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0.4, 0.3, 0.5)
SMILES Notation: CC[C@H](C)[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)CNC(=O)[C@@H](NC(=O)[C@@H](N)CC(C)C)[C@@H](C)CC)C(C)C)C(C)C)C(=O)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | EECCEEEEEEEEHTCHHHEE |
| Chou-Fasman (CF) | EEEEEEEEECCCEECCCCCC |
| Neural Network (NN) | CCCCEEEECCCHHHHHHHHH |
| Joint/Consensus | EECCEEEEECCCCCCCCCCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
Reference
1 : Mignogna G, et al. Antibacterial and haemolytic peptides containing D-alloisoleucine from the skin of Bombina variegata. EMBO J. 1993; 12:4829-32. doi: 10.1002/j.1460-2075.1993.tb06172.x
Literature
Paper title : Antibacterial and haemolytic peptides containing D-alloisoleucine from the skin of Bombina variegata.
Doi : https://doi.org/10.1002/j.1460-2075.1993.tb06172.x
Abstract : A family of bombinin-related peptides is present in the skin of Bombina variegata. These peptides contain 27 residues with Gly as N-terminus and display antimicrobial activity. From sequence analysis of the cDNAs encoding for the corresponding peptide precursors, the presence of a novel 20-residue peptide with Ile as N-terminus was predicted. We have now purified a family of hydrophobic peptides named H1-H5, whose sequences correspond to the predicted peptide with some variability in positions 1, 2 and 8. In particular, H3-H5 contain a D-alloisoleucine residue in the second position. All these peptides display antibacterial and haemolytic activity.