Peptide name : BPP-II

Source/Organism : Immunomodulatory bursal-derived Pentapeptide-II

Linear/Cyclic : Linear

Chirality : L

Peptide length: 5

C-terminal modification: Linear

N-terminal modification : Free

Non-natural peptide information: None

Assay type : MTT/MTS assay

Assay time : 48h

Activity : Not found

Cell line : CEF

Cancer type : Tumor

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 521.628 Dalton

Aliphatic index : 0.78

Instability index : -14.74

Hydrophobicity (GRAVY) : 0.78

Isoelectric point : 5.275

Charge (pH 7) : -0.4996

Aromaticity : 0

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 1.5

hydrophobic moment : -0.441

Missing amino acid : W,P,I,E,K,F,D,N,C,R,H,Q,S,Y,A,V

Most occurring amino acid : T

Most occurring amino acid frequency : 2

Least occurring amino acid : M

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0.4, 0.2, 0.6)

SMILES Notation: CSCC[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(=O)NCC(=O)O)[C@@H](C)O)[C@@H](C)O

1 : Feng XL, et al. Identification and characterization of novel immunomodulatory bursal-derived pentapeptide-II (BPP-II). J Biol Chem. 2012; 287:3798-807. doi: 10.1074/jbc.M111.273854

Paper title : Identification and characterization of novel immunomodulatory bursal-derived pentapeptide-II (BPP-II).

Doi : https://doi.org/10.1074/jbc.M111.273854

Abstract : The bursa of Fabricius, the acknowledged central humoral immune organ, plays a vital role in B lymphocyte differentiation. However, there are few reports of the molecular basis of the mechanism on immune induction and potential antitumor activity of bursal-derived peptides. In this paper, a novel bursal-derived pentapeptide-II (BPP-II, MTLTG) was isolated and exerted immunomodulatory functions on antibody responses in vitro. Gene microarray analyses demonstrated that BPP-II regulated expression of 2478 genes in a mouse-derived hybridoma cell line. Immune-related gene ontology functional procedures were employed for further functional analysis. Furthermore, the majority of BPP-II-regulated pathways were associated with immune responses and tumor processes. Moreover, BPP-II exhibited immunomodulatory effects on antigen-specific immune responses in vivo, including enhancement of avian influenza virus (H9N2 subtype)-specific antibody and cytokine production and modification of T cell immunophenotypes and lymphocyte proliferation. Finally, BPP-II triggered p53 expression and stabilization and selectively inhibited tumor cell proliferation. These data identified the multifunctional factor, BPP-II, as a novel biomaterial representing an important linking between the humoral central immune system and immune induction, including antitumor. Information generated in this study elucidates further the mechanisms involved in humoral immune system and represents the potential basis of effective immunotherapeutic strategies for treating human tumors and immune improvement.