Peptide name : Brevinin-1EMb

Source/Organism : Skin, Korean wrinkled frog, Asia

Linear/Cyclic : Not found

Chirality : Not found

Peptide length: 24

C-terminal modification: Not found

N-terminal modification : 2 invariant cysteine residues at the C-terminal

Non-natural peptide information: None

Assay type : Not specified

Assay time : Not found

Activity : Not found

Cell line : Not found

Cancer type : Not specified

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 2610.2265 Dalton

Aliphatic index : 1.425

Instability index : 15.8333

Hydrophobicity (GRAVY) : 1.2833

Isoelectric point : 8.8594

Charge (pH 7) : 1.7373

Aromaticity : 0.125

Molar extinction coefficient (cysteine, cystine): (1490, 1615)

Hydrophobic/hydrophilic ratio : 3

hydrophobic moment : -0.071

Missing amino acid : R,W,H,Q,M,E,D,V

Most occurring amino acid : L

Most occurring amino acid frequency : 5

Least occurring amino acid : G

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0.4, 0.2, 0.5)

SMILES Notation: CC[C@H](C)[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)[C@H](CC(C)C)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)Cc1ccccc1)[C@@H](C)O)[C@@H](C)CC)[C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)O

1 : Park JM, et al. Antimicrobial peptides from the skin of a Korean frog, Rana rugosa. Biochem Biophys Res Commun. 1994; 205:948-54. doi: 10.1006/bbrc.1994.2757

Paper title : Antimicrobial peptides from the skin of a Korean frog, Rana rugosa.

Doi : https://doi.org/10.1006/bbrc.1994.2757

Abstract : Six antimicrobial peptides, named gaegurins, were isolated from the skin of a Korean frog, Rana rugosa, and their amino acid sequences were determined by automated Edman degradation. All peptides contain two invariant cysteine residues, one at their C-terminus and the second at the seventh position from the C-terminus. The heptapeptides containing these two cysteine residues, which we designate 'Rana boxes', are conserved in the antimicrobial peptides derived from other Rana species. Each peptide manifested a broad spectrum of antimicrobial activity against Gram positive and Gram negative bacteria, fungi and protozoa with slightly different specific activities. All gaegurins manifest very little or no hemolytic activity. These properties provide the potential for application of these peptides to effective therapeutic agents for control of pathogenic microorganisms.