dbacp02009
General Description
Peptide name : Buforin IIb
Source/Organism : Histone H2A
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : RAGLQFPVGRLLRRLLRRLLR
Peptide length: 21
C-terminal modification: Linear
N-terminal modification : Free
Non-natural peptide information: None
Activity Information
Assay type : MTT/MTS assay
Assay time : 48h
Activity : IC50 : 10.7 µg/ml
Cell line : NCI-H322M
Cancer type : Lung cancer
Other activity : Not found
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 2560.1481 Dalton
Aliphatic index : 1.485
Instability index : 117.823
Hydrophobicity (GRAVY) : -0.095
Isoelectric point : 12
Charge (pH 7) : 6.76
Aromaticity : 0.047
Molar extinction coefficient (cysteine, cystine): (0, 0)
Hydrophobic/hydrophilic ratio : 1.625
hydrophobic moment : 1.3777
Missing amino acid : C,W,H,T,M,I,E,K,S,D,Y,N
Most occurring amino acid : R
Most occurring amino acid frequency : 7
Least occurring amino acid : A
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0.3, 0.1, 0.4)
SMILES Notation: CC(C)C[C@H](NC(=O)CNC(=O)[C@H](C)NC(=O)[C@@H](N)CCCNC(=N)N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N1CCC[C@H]1C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)O)C(C)C
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | HTTCCCCTTHHHHHHHHHHHH |
| Chou-Fasman (CF) | CCCCEEEEHHHHHHHHHHCCC |
| Neural Network (NN) | CCCCCCCHHHHHHHHHHHHHH |
| Joint/Consensus | CCCCCCCCHHHHHHHHHHHHH |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
Reference
1 : Lee HS, et al. Mechanism of anticancer activity of buforin IIb, a histone H2A-derived peptide. Cancer Lett. 2008; 271:47-55. doi: 10.1016/j.canlet.2008.05.041
Literature
Paper title : Mechanism of anticancer activity of buforin IIb, a histone H2A-derived peptide.
Doi : https://doi.org/10.1016/j.canlet.2008.05.041
Abstract : Buforin IIb is a novel cell-penetrating anticancer peptide derived from histone H2A. Here we analyzed the anticancer activity and cancer cell-killing mechanism of buforin IIb. Buforin IIb displayed selective cytotoxicity against 62 cancer cell lines by specifically targeting cancer cells through interaction with cell surface gangliosides. It traversed cancer cell membranes without damaging them and accumulated primarily in the nuclei. Once inside the cells, buforin IIb induced mitochondria-dependent apoptosis. In vivo analysis revealed that buforin IIb displayed significant tumor suppression activity in mice with tumor xenograft. Overall, these results suggest that buforin IIb constitutes a novel therapeutic agent for the treatment of cancers.