Peptide name : Buthus Occitanus RK1

Source/Organism : Common yellow scorpion RK1

Linear/Cyclic : Not found

Chirality : Not found

Peptide length: 14

C-terminal modification: Not found

N-terminal modification : Not found

Non-natural peptide information: None

Assay type : MTT assay, Cell proliferation assay

Assay time : 72h

Activity : MIC 50 : approx. 2 µM

Cell line : IGR39

Cancer type : EpCAM-expressing cells

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 1469.6373 Dalton

Aliphatic index : 0.835

Instability index : 48.3643

Hydrophobicity (GRAVY) : 0.1286

Isoelectric point : 4.3702

Charge (pH 7) : -1.2568

Aromaticity : 0

Molar extinction coefficient (cysteine, cystine): (0, 125)

Hydrophobic/hydrophilic ratio : 0.75

hydrophobic moment : 0.1648

Missing amino acid : R,W,H,Q,P,M,F,Y,G

Most occurring amino acid : S

Most occurring amino acid frequency : 3

Least occurring amino acid : I

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0.2, 0.3, 0.2)

SMILES Notation: CC[C@H](C)[C@H](N)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)O)[C@@H](C)O)C(C)C

1 : Khamessi O, et al. RK1, the first very short peptide from Buthus occitanus tunetanus inhibits tumor cell migration, proliferation and angiogenesis. Biochem Biophys Res Commun. 2018; 499:1-7. doi: 10.1016/j.bbrc.2018.01.133

Paper title : RK1, the first very short peptide from Buthus occitanus tunetanus inhibits tumor cell migration, proliferation and angiogenesis.

Doi : https://doi.org/10.1016/j.bbrc.2018.01.133

Abstract : Scorpion toxins have been the subject of many studies which explore their pharmacological potential toward diverse molecular targets, known to monitor key mechanisms in cancer such as proliferation, migration and angiogenesis. The few peptides from scorpion venom that have an anti-tumor effect are generally cytotoxic. Herein, we present the first description of a short 14 amino acid peptide (called RK1), purified from the venom of Buthus occitanus tunetanus, with the particular capabilities, among different other scorpion peptides, to inhibit cell proliferation, migration and angiogenesis of U87 (Glioblastoma) and IGR39 (Melanoma). Moreover, RK1 is a first peptide derived from scorpion venom exhibiting a potential anti-tumoral activity with no manifest toxicity. Our results suggest that, in terms of its primary structure, RK1 is unique compared to a variety of known peptides purified from scorpion venoms. In addition, RK1 is the first natural peptide able to abolish completely the proliferation of cancer cells. The Chicken chorioallantoic membrane model revealed that RK1 strongly inhibits ex-vivo vascular growth. RK1 could open new perspective for the pharmaceutical application of short scorpion venom peptides in anticancer activity and may represent the first member of a new group of scorpion peptides.