dbacp02473
General Description
Peptide name : Chickpea peptide
Source/Organism : Plant sources
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : ADLPGLK
Peptide length: 7
C-terminal modification: Linear
N-terminal modification : Not found
Non-natural peptide information: None
Activity Information
Assay type : SRB assay
Assay time : 1h
Activity : IC50 : 113.7 ± 0.99 µM
Cell line : HepG-2
Cancer type : Human endometrial cancer
Other activity : Not found
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 712.8346 Dalton
Aliphatic index : 1.257
Instability index : 11.8286
Hydrophobicity (GRAVY) : 0
Isoelectric point : 5.8804
Charge (pH 7) : -0.204
Aromaticity : 0
Molar extinction coefficient (cysteine, cystine): (0, 0)
Hydrophobic/hydrophilic ratio : 2.5
hydrophobic moment : -0.461
Missing amino acid : C,R,W,H,Q,T,M,I,E,F,S,Y,N,V
Most occurring amino acid : L
Most occurring amino acid frequency : 2
Least occurring amino acid : A
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0.5, 0.4, 0.2)
SMILES Notation: CC(C)C[C@H](NC(=O)CNC(=O)[C@@H]1CCCN1C(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](C)N)C(=O)N[C@@H](CCCCN)C(=O)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | CCCTTCC |
| Chou-Fasman (CF) | CCCCCCC |
| Neural Network (NN) | CCCCCCC |
| Joint/Consensus | CCCCCCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
CancerPPD : Not available
ApIAPDB : Not available
CancerPPD2 ID : Not available
Reference
1 : Gupta N and Bhagyawant SS. Bioactive peptide of Cicer arietinum L. induces apoptosis in human endometrial cancer via DNA fragmentation and cell cycle arrest. 3 Biotech. 2021; 11:63. doi: 10.1007/s13205-020-02614-6
Literature
Paper title : Bioactive peptide of Cicer arietinum L. induces apoptosis in human endometrial cancer via DNA fragmentation and cell cycle arrest.
Doi : https://doi.org/10.1007/s13205-020-02614-6
Abstract : Chickpea seed proteins are alleged source of nutraceuticals. These seed proteins were subjected to different proteases to produce peptides. The efficacy of these peptides was confirmed using six diverse human cancer cell lines (PA-1, Ishikawa cells, A549, MCF-7, HepG2, MDA-MB-231). Alcalase generated peptides exhibited the highest antagonistic inhibition of Ishikawa cells. Flow cytometric analysis revealed that chickpea peptide induced S and G2 phase arrest of cell cycle in a dose dependent manner. DNA fragmentation and apoptosis occurred by down regulation of Bcl-2 expression, upregulation of Bax expression and promotion of caspase-3 initiation. Chickpea peptides ascertain potential antiproliferative molecule that can be deployed in cancer treatment regimes.