Peptide name : Cn-AMP1

Source/Organism : Green coconut water

Linear/Cyclic : Not found

Chirality : L

Peptide length: 9

C-terminal modification: Not found

N-terminal modification : Free

Non-natural peptide information: None

Assay type : Not specified

Assay time : Not found

Activity : Not found

Cell line : Not found

Cancer type : Not found

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 875.9929 Dalton

Aliphatic index : 0.544

Instability index : 8.8889

Hydrophobicity (GRAVY) : 0.0111

Isoelectric point : 9.4651

Charge (pH 7) : 0.4601

Aromaticity : 0

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 2

hydrophobic moment : -0.727

Missing amino acid : C,W,H,T,P,I,E,K,F,D,Y,L,N

Most occurring amino acid : A

Most occurring amino acid frequency : 2

Least occurring amino acid : S

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0.3, 0.3, 0.1)

SMILES Notation: CSCC[C@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@@H](N)CO)C(C)C)C(=O)O

1 : Mandal SM, et al. Identification and structural insights of three novel antimicrobial peptides isolated from green coconut water. Peptides. 2009; 30:633-7. doi: 10.1016/j.peptides.2008.12.001

Paper title : Identification and structural insights of three novel antimicrobial peptides isolated from green coconut water.

Doi : https://doi.org/10.1016/j.peptides.2008.12.001

Abstract : Infections caused by pathogenic bacteria could cause an expressive negative impact on human health. A significant enhance in resistance to commercial antibiotics has been observed in all kinds of pathogenic bacteria. In order to find novel approaches to control such common infections, a wide number of defense peptides with bactericidal properties have been characterized. In this report, three peptides lower than 3kDa were purified and identified from green coconut (Cocos nucifera L.) water by using reversed phase-high performance liquid chromatography (HPLC), showing molecular masses of 858Da, 1249Da and 950Da. First one, named Cn-AMP1, was extremely efficient against both Gram-positive and Gram-negative bacteria, being MICs calculated for three peptides. All complete sequences were determined by MALDI-ToF analysis showing no identity in databanks. Moreover, peptide net charge and hydrophobicity of each peptide was in silico evaluated. Finally molecular modeling and dynamics were also applied generating peptides three-dimensional structures, indicating a better explanation to probable mechanisms of action. Cn-AMPs here reported show remarkable potential to contribute in the development of novel antibiotics from natural sources.