Peptide name : CSP-KQ

Source/Organism : Not found

Linear/Cyclic : Cyclic

Chirality : Not found

Peptide length: 7

C-terminal modification: Cyclic

N-terminal modification : Not found

Non-natural peptide information: None

Assay type : Not specified

Assay time : Not found

Activity : Not found

Cell line : C-33A

Cancer type : Human Cervical cancer

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 758.8202 Dalton

Aliphatic index : 0.7

Instability index : 42.3429

Hydrophobicity (GRAVY) : -1.314

Isoelectric point : 6.0014

Charge (pH 7) : -0.2381

Aromaticity : 0

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 0.75

hydrophobic moment : 0.8795

Missing amino acid : C,R,W,H,T,P,M,I,F,S,D,Y,V

Most occurring amino acid : K

Most occurring amino acid frequency : 1

Least occurring amino acid : K

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0.5, 0.2, 0.1)

SMILES Notation: CC(C)C[C@H](NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(=O)O)C(=O)NCC(=O)O

1 : Liu X, et al. Selection and identification of novel peptides specifically targeting human cervical cancer. Amino Acids. 2018; 50:577-592. doi: 10.1007/s00726-018-2539-1

Paper title : Selection and identification of novel peptides specifically targeting human cervical cancer.

Doi : https://doi.org/10.1007/s00726-018-2539-1

Abstract : Cervical cancer is the second most commonly diagnosed cancer and the third leading cause of cancer deaths among females in underdeveloped countries. This study aimed to identify several novel cervical cancer-specific targeting peptides (CSPs) to provide new methods for the effective diagnosis and treatment of cervical cancer. Peptide library screening in vivo was performed on human cervical cancer xenografts with Ph.D.™-12 and C7C phage display peptide libraries. Two specific peptide sequences (GDALFSVPLEVY and KQNLAEG), which were enriched in tumors, were screened, and respectively, named CSP-GD and CSP-KQ through three rounds of biopanning. The in vivo tumor-targeting ability of these peptides was identified by injecting them into mice with cervical cancer xenograft. CSPs were compounded and labeled with fluorescein isothiocyanate (FITC). The specificity and affinity of FITC-CSPs were evaluated in human cervical cancer cell lines and tissue microarrays in vitro by immunofluorescent staining. Results showed that FITC-CSP-GD and FITC-CSP-KQ evidently and specifically bound to the cell membrane and cytoplasm of SiHa, ME-180, and C-33A cells in vitro. In human cervical cancer tissue, FITC-CSP-GD and FITC-CSP-KQ strongly targeted human cervical adenocarcinoma and cervical squamous cell carcinoma tissues, respectively. A bright FITC signal was located mainly on the cell membrane and cytoplasm of tumor cells. In conclusion, the novel 12-residue peptide CSP-GD and 7-residue peptide CSP-KQ could specifically target human cervical cancer and may have the potential to be used in the diagnosis and targeted therapy of cervical cancer.