Peptide name : D-K6L9

Source/Organism : Not found

Linear/Cyclic : Linear

Chirality : Mix

Peptide length: 15

C-terminal modification: Linear

N-terminal modification : Amidation

Non-natural peptide information: None

Assay type : Enzyme Immunoassay (EIA)

Assay time : Not found

Activity : LC50 : 3 µMol/L

Cell line : MB-231

Cancer type : Prostate cancer

Other activity : Anti-microbial activity

Amino acid composition bar chart :

Molecular mass : 1805.4675 Dalton

Aliphatic index : 1.82

Instability index : -35.946

Hydrophobicity (GRAVY) : 0.72

Isoelectric point : 10.699

Charge (pH 7) : 5.7541

Aromaticity : 0

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 1.75

hydrophobic moment : 0.8405

Missing amino acid : W,T,P,I,M,E,F,D,N,G,C,R,H,Q,S,Y,A,V

Most occurring amino acid : L

Most occurring amino acid frequency : 7

Least occurring amino acid : l

Least occurring amino acid frequency : 2

Secondary structure fraction (Helix, Turn, Sheet): (1, 0, 0.6)

SMILES Notation: CC(C)C[C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CC(C)C)C(=O)O

1 : Papo N, et al. Inhibition of tumor growth and elimination of multiple metastases in human prostate and breast xenografts by systemic inoculation of a host defense-like lytic peptide. Cancer Res. 2006; 66:5371-8. doi: 10.1158/0008-5472.CAN-05-4569

Paper title : Inhibition of tumor growth and elimination of multiple metastases in human prostate and breast xenografts by systemic inoculation of a host defense-like lytic peptide.

Doi : https://doi.org/10.1158/0008-5472.CAN-05-4569

Abstract : We report on a short host defense-like peptide that targets and arrests the growth of aggressive and hormone-resistant primary human prostate and breast tumors and prevents their experimental and spontaneous metastases, respectively, when systemically inoculated to immunodeficient mice. These effects are correlated with increased necrosis of the tumor cells and a significant decrease in the overall tumor microvessel density, as well as newly formed capillary tubes and prostate-specific antigen secretion (in prostate tumors). Growth inhibition of orthotopic tumors derived from stably transfected highly fluorescent human breast cancer cells and prevention of their naturally occurring metastases were visualized in real time by using noninvasive whole-body optical imaging. The exclusive selectivity of the peptide towards cancer derives from its specific binding to surface phosphatidylserine and the killing of the cancer cells via cytoplasmic membrane depolarization. These data indicate that membrane disruption can provide a therapeutic means of inhibiting tumor growth and preventing metastases of various cancers.