dbacp03335
General Description
Peptide name : Human lactoferricin
Source/Organism : Milk, Human
Linear/Cyclic : Linear
Chirality : Not found
Sequence Information
Sequence : GRRRRSVQWCAVSQPEATKCFQWQRnMRKVRGPPVSCIKRDSPIQCIQA
Peptide length: 49
C-terminal modification: Linear
N-terminal modification : Free
Non-natural peptide information: None
Activity Information
Assay type : Not specified
Assay time : 48h
Activity : MIC : >200µg/ml
Cell line : Not found
Cancer type : Not found
Other activity : Anti- microbial activity
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 5743.6652 Dalton
Aliphatic index : 0.536
Instability index : 93.549
Hydrophobicity (GRAVY) : -0.851
Isoelectric point : 11.240
Charge (pH 7) : 8.7214
Aromaticity : 0.061
Molar extinction coefficient (cysteine, cystine): (11000, 11250)
Hydrophobic/hydrophilic ratio : 1
hydrophobic moment : -0.118
Missing amino acid : N,Y,H,L
Most occurring amino acid : R
Most occurring amino acid frequency : 8
Least occurring amino acid : E
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0.1, 0.2, 0.2)
SMILES Notation: CC[C@H](C)[C@H](NC(=O)[C@H](CS)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)[C@H](CO)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](CS)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)[C@@H]1CCCN1C(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCSC)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CS)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CS)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCNC(=N)N)NC(=O)CN)C(C)C)C(C)C)[C@@H](C)O)C(C)C)C(C)C)[C@@H](C)CC)[C@@H](C)CC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C)C(=O)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | HHEEEEEEEEHTTCCHHHHHHHHHHHHTEETCCCCEEEECCCCCEEEHH |
| Chou-Fasman (CF) | CCCEEEECEEEECHHHHCCEECCHHHHEEECCCEEEECCCCEEEECCCC |
| Neural Network (NN) | CCCCCCEEEECCCCCCCCHHHHHHHCCCCCCCCCCCECCCCCCCEEEEC |
| Joint/Consensus | CCCEEEEEEECCCCCCCCHHHHHHHHHCEECCCCCEECCCCCCCEEECC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
CancerPPD : Not available
ApIAPDB : Not available
CancerPPD2 ID : Not available
Reference
1 : Vorland LH, et al. Lactoferricin of bovine origin is more active than lactoferricins of human, murine and caprine origin. Scand J Infect Dis. 1998; 30:513-7. doi: 10.1080/00365549850161557
Literature
Paper title : Lactoferricin of bovine origin is more active than lactoferricins of human, murine and caprine origin.
Doi : https://doi.org/10.1080/00365549850161557
Abstract : The antimicrobial peptide lactoferricin is generated by gastric pepsin cleavage of lactoferrin. We have examined the antimicrobial activity of lactoferricins derived from lactoferrin of human, murine, caprine and bovine origin with minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against E. coli ATCC 25922 and S. aureus ATCC 25923. We found that lactoferricin of bovine origin (Lf-cin B) was the most efficacious of the lactoferricins tested. By comparing the linear and cyclic Lf-cin B we found the cyclic peptide to be the most active. Lactoferricin B was moderately active against E. coli ATCC 25922 and S. aureus ATCC 25923, but had no activity against P. mirabilis or Y. enterocolitica. Lf-cin B showed good activity against C. albicans, C. tropicalis and C. neoformans.