Peptide name : IbACP

Source/Organism : Sweet potato leaves

Linear/Cyclic : linear

Chirality : Not found

Peptide length: 16

C-terminal modification: linear

N-terminal modification : Not found

Non-natural peptide information: None

Assay type : MTT/MTS assay

Assay time : 24h

Activity : MIC : 5 mg/ml

Cell line : H1299

Cancer type : Lung cancer

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 1597.735 Dalton

Aliphatic index : 0.55

Instability index : 57.3125

Hydrophobicity (GRAVY) : -0.85

Isoelectric point : 11.699

Charge (pH 7) : 1.797

Aromaticity : 0

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 1.28571428

hydrophobic moment : 0.4769

Missing amino acid : C,W,H,M,I,E,K,F,Y,N

Most occurring amino acid : G

Most occurring amino acid frequency : 4

Least occurring amino acid : S

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0.1, 0.4, 0.1)

SMILES Notation: CC(C)C[C@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)CNC(=O)CNC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](C)NC(=O)[C@H](C)N)[C@@H](C)O)C(C)C)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)O

1 : Chang VH, et al. IbACP, a sixteen-amino-acid peptide isolated from Ipomoea batatas leaves, induces carcinoma cell apoptosis. Peptides. 2013; 47:148-56. doi: 10.1016/j.peptides.2013.02.005

Paper title : IbACP, a sixteen-amino-acid peptide isolated from Ipomoea batatas leaves, induces carcinoma cell apoptosis.

Doi : https://doi.org/10.1016/j.peptides.2013.02.005

Abstract : A 16-amino-acid peptide was isolated from the leaves of sweet potato. The peptide caused a rapid alkalinization response in tomato suspension culture media, a characteristic of defense peptides in plants. No post-translational modification was observed on the peptide according to MALDI-MS analysis. We have named the peptide Ipomoea batatas anti-cancer peptide (IbACP). IbACP also was shown with the ability to dose-dependently inhibit Panc-1, a pancreatic cancer line, cell proliferation. The morphological observations of the Panc-1 cells by phase contrast microscopy showed significant changes after treatment with IbACP. Moreover, caspase-3 and PARP [poly(ADP-ribose) polymerase] were activated by IbACP treatment, followed by cell death. An increase in the levels of cleaved caspase-3 and -9 was also detected by an immunoblot assay after treatment with IbACP. In addition, genomic DNA fragmentation and decreased cellular proliferation were induced when IbACP was supplied to the Panc-1 cells, further demonstrating its biological relevance. The combined data indicates that IbACP peptide may have an important role in the regulation of cellular proliferation by inducing and promoting apoptosis through the mitochondrial apoptotic pathway. This report also showed that IbACP peptide contains potent anti-cancer effects and may play an important role in herbal medicine development.