Peptide name : l3,10,13K7,8K4R2L9

Source/Organism : Diastereomeric peptide

Linear/Cyclic : Linear

Chirality : Mix

Peptide length: 15

C-terminal modification: Linear

N-terminal modification : Free

Non-natural peptide information: None

Assay type : XTT assay

Assay time : 24h

Activity : LC50 : 4.5 µM

Cell line : D-122

Cancer type : Lung cancer

Other activity : Anti-microbial activity

Amino acid composition bar chart :

Molecular mass : 1861.4943 Dalton

Aliphatic index : 1.56

Instability index : 12.3733

Hydrophobicity (GRAVY) : 0.64

Isoelectric point : 12

Charge (pH 7) : 5.7561

Aromaticity : 0

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 1.5

hydrophobic moment : -0.617

Missing amino acid : C,W,H,Q,T,P,M,I,E,F,S,D,Y,N,A,V,G

Most occurring amino acid : L

Most occurring amino acid frequency : 6

Least occurring amino acid : K

Least occurring amino acid frequency : 2

Secondary structure fraction (Helix, Turn, Sheet): (0.8, 0, 0.6)

SMILES Notation: CC(C)C[C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O

1 : Papo N, et al. A novel lytic peptide composed of DL-amino acids selectively kills cancer cells in culture and in mice. J Biol Chem. 2003; 278:21018-23. doi: 10.1074/jbc.M211204200

Paper title : A novel lytic peptide composed of DL-amino acids selectively kills cancer cells in culture and in mice.

Doi : https://doi.org/10.1074/jbc.M211204200

Abstract : The high toxicity of most chemotherapeutic drugs and their inactivation by multidrug resistance phenotypes motivated extensive search for drugs with new modes of action. We designed a short cationic diastereomeric peptide composed of d- and l-leucines, lysines, and arginines that has selective toxicity toward cancer cells and significantly inhibits lung metastasis formation in mice (86%) with no detectable side effects. Its ability to depolarize the transmembrane potential of cancer cells at the same rate (within minutes) and concentration (3 micro m), at which it shows biological activity, suggests a killing mechanism that involves plasma membrane perturbation. Confocal microscopy experiments verified that the cells died as a result of acute injury, swelling, and bursting, suggesting necrosis. Biosensor binding experiments and attenuated total reflectance-Fourier transform infrared spectroscopy using model membranes have substantiated its high selectivity toward cancer cells. Although this is an initial study that looked at tumor formation rather than the ability of the peptides to reduce established tumors, the simple sequence of the peptide, its high solubility, substantial resistance to degradation, and inactivation by serum components might make it a good candidate for future anticancer treatment.