Peptide name : Malanin chain A

Source/Organism : Plant sources

Linear/Cyclic : Linear

Chirality : L

Peptide length: 10

C-terminal modification: Linear

N-terminal modification : Not found

Non-natural peptide information: None

Assay type : MTT assay

Assay time : Not found

Activity : IC50 : 7.71 ± 0.24 nM

Cell line : PC-12

Cancer type : Breast cancer

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 1172.2838 Dalton

Aliphatic index : 0.39

Instability index : 25.19

Hydrophobicity (GRAVY) : -0.66

Isoelectric point : 5.8349

Charge (pH 7) : -0.2408

Aromaticity : 0.2

Molar extinction coefficient (cysteine, cystine): (1490, 1490)

Hydrophobic/hydrophilic ratio : 0.42857142

hydrophobic moment : -0.165

Missing amino acid : C,R,W,H,Q,M,I,E,N,A,V,G

Most occurring amino acid : T

Most occurring amino acid frequency : 3

Least occurring amino acid : D

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0.2, 0.3, 0.6)

SMILES Notation: CC(C)C[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@@H]1CCCN1C(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](N)CC(=O)O)C(=O)N[C@H](C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)O)[C@@H](C)O)[C@@H](C)O)[C@@H](C)O

1 : Yuan Y, et al. Purification, characterization and cytotoxicity of malanin, a novel plant toxin from the seeds of Malania oleifera. Toxicon. 2009; 54:121-7. doi: 10.1016/j.toxicon.2009.03.024

Paper title : Purification, characterization and cytotoxicity of malanin, a novel plant toxin from the seeds of Malania oleifera.

Doi : https://doi.org/10.1016/j.toxicon.2009.03.024

Abstract : Malanin, a novel plant toxin with a molecular weight of 61,875 Da and an isoelectric point of 5.5, was isolated from Malania oleifera seeds by homogenization, ammonium sulfate precipitation and hydrophobic interaction chromatography (HIC). It is a glycoprotein with two chains, chain-A and chain-B, which are crosslinked by one or more disulfide bonds. The N-terminal amino-acid sequences of malanin are DETXTDEEFN (X was commonly C) in chain-B, and DYPKLTFTTS in chain-A. Malanin exhibited highly cytotoxic activities against cancer cell lines (HeLa, PC-12, MCF-7, K562) and non-cancer cell lines (Vero and MDCK), producing IC(50) values of 0.15+/-0.08, 7.71+/-0.24, 11.20+/-0.02, 15.80+/-0.09, 2.79+/-0.05 and 3.92+/-0.01 nM, respectively. It significantly inhibited the growth of HeLa cells through cell-cycle arrest at S phase and induced an apoptotic response. LD(50) values were determined in ICR mice, which were found to be 26.22 microg/kg and 43.11 mg/kg by i.p. and i.g. respectively. Thus, malanin is amongst the most potent toxin of plant origin.