Peptide name : Malanin chain B

Source/Organism : Plant sources

Linear/Cyclic : Linear

Chirality : L

Peptide length: 10

C-terminal modification: Linear

N-terminal modification : Not found

Non-natural peptide information: None

Assay type : MTT assay

Assay time : Not found

Activity : IC50 : 11.20 ± 0.02 nM

Cell line : MCF-7

Cancer type : Leukemia

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 1202.1592 Dalton

Aliphatic index : 0

Instability index : 74.2

Hydrophobicity (GRAVY) : -1.71

Isoelectric point : 4.05

Charge (pH 7) : -5.2391

Aromaticity : 0.1

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 0.25

hydrophobic moment : 0.3608

Missing amino acid : R,W,H,Q,P,M,I,K,S,Y,L,A,V,G

Most occurring amino acid : E

Most occurring amino acid frequency : 3

Least occurring amino acid : C

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0.3, 0.3, 0.3)

SMILES Notation: C[C@@H](O)[C@H](NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H](N)CC(=O)O)[C@@H](C)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(N)=O)C(=O)O

1 : Yuan Y, et al. Purification, characterization and cytotoxicity of malanin, a novel plant toxin from the seeds of Malania oleifera. Toxicon. 2009; 54:121-7. doi: 10.1016/j.toxicon.2009.03.024

Paper title : Purification, characterization and cytotoxicity of malanin, a novel plant toxin from the seeds of Malania oleifera.

Doi : https://doi.org/10.1016/j.toxicon.2009.03.024

Abstract : Malanin, a novel plant toxin with a molecular weight of 61,875 Da and an isoelectric point of 5.5, was isolated from Malania oleifera seeds by homogenization, ammonium sulfate precipitation and hydrophobic interaction chromatography (HIC). It is a glycoprotein with two chains, chain-A and chain-B, which are crosslinked by one or more disulfide bonds. The N-terminal amino-acid sequences of malanin are DETXTDEEFN (X was commonly C) in chain-B, and DYPKLTFTTS in chain-A. Malanin exhibited highly cytotoxic activities against cancer cell lines (HeLa, PC-12, MCF-7, K562) and non-cancer cell lines (Vero and MDCK), producing IC(50) values of 0.15+/-0.08, 7.71+/-0.24, 11.20+/-0.02, 15.80+/-0.09, 2.79+/-0.05 and 3.92+/-0.01 nM, respectively. It significantly inhibited the growth of HeLa cells through cell-cycle arrest at S phase and induced an apoptotic response. LD(50) values were determined in ICR mice, which were found to be 26.22 microg/kg and 43.11 mg/kg by i.p. and i.g. respectively. Thus, malanin is amongst the most potent toxin of plant origin.