dbacp04651
General Description
Peptide name : Melittin
Source/Organism : Natural component of bee venom
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : GIGAVLKVLTTGLPALISWIKRKRQQ
Peptide length: 26
C-terminal modification: Linear
N-terminal modification : Amidation
Non-natural peptide information: None
Activity Information
Assay type : MTT/MTS assay
Assay time : 24h
Activity : IC50 : 1.4 ± 0.1 µM
Cell line : E42/02
Cancer type : Skin cancer
Other activity : Hemolytic activity
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 2847.4471 Dalton
Aliphatic index : 1.35
Instability index : 44.7308
Hydrophobicity (GRAVY) : 0.2731
Isoelectric point : 12
Charge (pH 7) : 4.7571
Aromaticity : 0.038
Molar extinction coefficient (cysteine, cystine): (5500, 5500)
Hydrophobic/hydrophilic ratio : 1.6
hydrophobic moment : 0.1665
Missing amino acid : C,H,M,E,F,D,Y,N
Most occurring amino acid : L
Most occurring amino acid frequency : 4
Least occurring amino acid : P
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0.3, 0.1, 0.4)
SMILES Notation: CC[C@H](C)[C@H](NC(=O)CN)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)O)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)[C@@H](C)O)C(C)C)C(C)C
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | CCEEEEEEEEECCCHHHHHHHHHHHH |
| Chou-Fasman (CF) | EEECCCEEEECCCCEEEEEHHHHCCC |
| Neural Network (NN) | CCCEEEEEHCCCCCCHHHHHHCCCCC |
| Joint/Consensus | CCEEEEEEEECCCCCHHHHHHHHCCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
Reference
1 : Gross S, et al. Design of NK-2-derived peptides with improved activity against equine sarcoid cells. J Pept Sci. 2013; 19:619-28. doi: 10.1002/psc.2540
Literature
Paper title : Design of NK-2-derived peptides with improved activity against equine sarcoid cells.
Doi : https://doi.org/10.1002/psc.2540
Abstract : Equine sarcoid is a topically accessible model for the evaluation of anticancer peptides acting by physical membrane disruption avoiding the complexity of a systemic application. We aim at evaluating and improving natural peptides for host defence as lead structures, where we focus on the cationic and amphipathic peptide NK-2. Cytotoxicity tests, fluorescence microscopy and a chip-based biosensor, which enabled real-time monitoring of cell metabolism, were applied. Cancer cell killing was dynamic with an initial phase of increased cellular respiration, followed by membrane destruction. NK-2 was substantially improved and shortened. Novel peptides exhibited a fivefold improved activity against sarcoid cells, while haemolysis remained almost unaltered. Similar Zeta potential and similar amount of surface phosphatidylserine of sarcoid and normal skin cells are responsible for a lack of selectivity between these two cell types.