dbacp04848
General Description
Peptide name : Nisin Z
Source/Organism : Mesophilic lactic NIZO 221 86
Linear/Cyclic : Not found
Chirality : Not found
Sequence Information
Sequence : ITSISLCTPGCKTGALMGCnMKTATCNCSIHVSK
Peptide length: 34
C-terminal modification: Not found
N-terminal modification : Not found
Non-natural peptide information: None
Activity Information
Assay type : Not specified
Assay time : Not found
Activity : Not found
Cell line : Not found
Cancer type : Not found
Other activity : Anti- microbial activity
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 3475.1558 Dalton
Aliphatic index : 0.717
Instability index : 17.0824
Hydrophobicity (GRAVY) : 0.4059
Isoelectric point : 8.7803
Charge (pH 7) : 2.7948
Aromaticity : 0
Molar extinction coefficient (cysteine, cystine): (0, 250)
Hydrophobic/hydrophilic ratio : 1.35714285
hydrophobic moment : -0.069
Missing amino acid : R,W,Q,E,F,D,Y
Most occurring amino acid : T
Most occurring amino acid frequency : 5
Least occurring amino acid : P
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0.2, 0.2, 0.3)
SMILES Notation: CC[C@H](C)[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CS)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)O)C(C)C)[C@@H](C)CC)[C@@H](C)O)[C@@H](C)O)[C@@H](C)O)[C@@H](C)O)[C@@H](C)CC)[C@@H](C)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | EEEEEEECTTCCCTCEETCCCTTTCTTTTEEETT |
| Chou-Fasman (CF) | EEEEEECCCEECCCCCCCHHHHCCCCEEEEECCC |
| Neural Network (NN) | EEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCC |
| Joint/Consensus | EEEEEECCCCCCCCCCCCCCCCCCCCCCCEECCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
CancerPPD : Not available
ApIAPDB : Not available
CancerPPD2 ID : Not available
Reference
1 : Mulders JW, et al. Identification and characterization of the lantibiotic nisin Z, a natural nisin variant. Eur J Biochem. 1991; 201:581-4. doi: 10.1111/j.1432-1033.1991.tb16317.x
Literature
Paper title : Identification and characterization of the lantibiotic nisin Z, a natural nisin variant.
Doi : https://doi.org/10.1111/j.1432-1033.1991.tb16317.x
Abstract : Lactococcus lactis strain NIZO 22186 produces an extracellular, lanthionine-containing 3.5-kDa polypeptide with antimicrobial activity. Its retention time on reversed-phase (RP) HPLC and its amino acid composition showed high similarities but no complete identity to nisin. The gene for this lantibiotic, designated nisZ, has been cloned and its nucleotide sequence was found to be identical to that of the precursor nisin gene apart from a single mutation resulting in the substitution His27Asn in the mature polypeptide. NMR studies of the natural nisin variant, which has been designated nisin Z, confirmed the His27Asn substitution and indicated that it has a similar structure to nisin.