dbacp04867
General Description
Peptide name : NK-2
Source/Organism : NK-lysin
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : KILRGVCKKIMRTFLRRISKDILTGKK
Peptide length: 27
C-terminal modification: Linear
N-terminal modification : Amidation
Non-natural peptide information: None
Activity Information
Assay type : PI-uptake assay
Assay time : Not found
Activity : LD50 : 1-4 µM
Cell line : SW-480
Cancer type : Leukemia cancer
Other activity : Not found
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 3203.0139 Dalton
Aliphatic index : 1.118
Instability index : 45.4333
Hydrophobicity (GRAVY) : -0.263
Isoelectric point : 11.604
Charge (pH 7) : 8.7453
Aromaticity : 0.037
Molar extinction coefficient (cysteine, cystine): (0, 0)
Hydrophobic/hydrophilic ratio : 0.92857142
hydrophobic moment : -0.777
Missing amino acid : W,H,Q,P,E,Y,N,A
Most occurring amino acid : K
Most occurring amino acid frequency : 6
Least occurring amino acid : V
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0.3, 0.1, 0.4)
SMILES Notation: CC[C@H](C)[C@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCSC)NC(=O)[C@@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@@H](N)CCCCN)[C@@H](C)CC)C(C)C)[C@@H](C)CC)[C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)[C@@H](C)O)[C@@H](C)CC
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | HHHHHHHHHHHHHHHHHHHTHHHHTCC |
| Chou-Fasman (CF) | CEEEEHHHHEEEECEEECCCEEECCCC |
| Neural Network (NN) | HHHHHHHHHHHHHHHCCCCCCCCCCCC |
| Joint/Consensus | HHHHHHHHHHHHHHHCCCCCCCCCCCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
Reference
1 : Schröder-Borm H, et al. The NK-lysin derived peptide NK-2 preferentially kills cancer cells with increased surface levels of negatively charged phosphatidylserine. FEBS Lett. 2005; 579:6128-34. doi: 10.1016/j.febslet.2005.09.084
Literature
Paper title : The NK-lysin derived peptide NK-2 preferentially kills cancer cells with increased surface levels of negatively charged phosphatidylserine.
Doi : https://doi.org/10.1016/j.febslet.2005.09.084
Abstract : The NK-lysin derived peptide NK-2 is a potent antibacterial, but non-toxic to a human keratinocyte cell line and of low hemolytic activity. Its target selectivity is based upon a strong binding preference to membranes containing anionic phospholipids, which are normally not found on the surface of human cells. Here, we analyzed the interaction of NK-2 with normal human lymphocytes and seven different human cancer cell lines and demonstrate that some of these cells expose negatively charged surface phosphatidylserine (PS), which presumably facilitates killing of the cells by NK-2. This is underlined by the specific intercalation of the peptide into PS-containing liposomes analyzed by fluorescence-resonance energy transfer spectroscopy.