Peptide name : Non-digestible fraction peptide

Source/Organism : Common bean

Linear/Cyclic : Not found

Chirality : Not found

Peptide length: 7

C-terminal modification: Not found

N-terminal modification : Not found

Non-natural peptide information: None

Assay type : MTS assay

Assay time : 24h

Activity : IC50 : 0.51 mg/ml

Cell line : HCT116

Cancer type : Colorectal cancer

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 651.7532 Dalton

Aliphatic index : 0.142

Instability index : 189.142

Hydrophobicity (GRAVY) : 0.3714

Isoelectric point : 5.2727

Charge (pH 7) : -0.5095

Aromaticity : 0

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 2.5

hydrophobic moment : 0.349

Missing amino acid : R,W,H,Q,T,I,E,K,F,D,Y,L,N,V

Most occurring amino acid : S

Most occurring amino acid frequency : 2

Least occurring amino acid : M

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0.2, 0.5, 0)

SMILES Notation: CSCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](CS)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)O

1 : Luna Vital DA, et al. Peptides in common bean fractions inhibit human colorectal cancer cells. Food Chem. 2014; 157:347-55. doi: 10.1016/j.foodchem.2014.02.050

Paper title : Peptides in common bean fractions inhibit human colorectal cancer cells.

Doi : https://doi.org/10.1016/j.foodchem.2014.02.050

Abstract : The aim of this study was to characterize peptides present in common bean non-digestible fractions (NDF) produced after enzymatic digestion and determine their antiproliferative action on human colorectal cancer cells. Five NDF peptides represented 70% of total protein (GLTSK, LSGNK, GEGSGA, MPACGSS and MTEEY) with antiproliferative activity on human colon cancer cells. Based on the antiproliferative effect, HCT116 cell line was most sensitive to bean Azufrado Higuera (IC50=0.53 mg/ml) and RKO to Bayo Madero (IC50=0.51 mg/ml) peptide extracts. Both cultivars increased significantly (p<0.05) the expression of p53 in HCT116 by 76% and 68%, respectively. Azufrado Higuera modified the expression of cell cycle regulation proteins p21 and cyclin B1. Bayo Madero modified the expression of mitochondrial activated apoptotic proteins BAD, cytC, c-casp3, Survivin, BIRC7. Results suggest that peptides present in common bean NDF contributed to the antiproliferative effect on human colorectal cancer cells by modifying molecules involved in either cell cycle arrest or apoptosis.