dbacp05040
General Description
Peptide name : P18
Source/Organism : Synthetic peptide
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : KWKLFKKIPKFLHLAKKF
Peptide length: 18
C-terminal modification: Linear
N-terminal modification : Free
Non-natural peptide information: None
Activity Information
Assay type : MTT/MTS assay
Assay time : Not found
Activity : 50 ± 3.09% cell viability at 40 µM
Cell line : M14
Cancer type : Skin cancer
Other activity : Not found
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 2300.9155 Dalton
Aliphatic index : 0.922
Instability index : -9.9389
Hydrophobicity (GRAVY) : -0.383
Isoelectric point : 10.778
Charge (pH 7) : 6.8403
Aromaticity : 0.222
Molar extinction coefficient (cysteine, cystine): (5500, 5500)
Hydrophobic/hydrophilic ratio : 1.25
hydrophobic moment : 1.2282
Missing amino acid : C,R,Q,T,M,E,S,D,Y,N,V,G
Most occurring amino acid : K
Most occurring amino acid frequency : 7
Least occurring amino acid : W
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0.6, 0.0, 0.4)
SMILES Notation: CC[C@H](C)[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@@H](N)CCCCN)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | HHHHHHHHHHHHHHHHHH |
| Chou-Fasman (CF) | HHHHHCCCHHHHHHHCCC |
| Neural Network (NN) | HHHHHCHHHHHHHHHHHH |
| Joint/Consensus | HHHHHCHHHHHHHHHHHH |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
Reference
1 : Huang W, et al. Anti-melanoma activity of hybrid peptide P18 and its mechanism of action. Biotechnol Lett. 2010; 32:463-9. doi: 10.1007/s10529-009-0175-2
Literature
Paper title : Anti-melanoma activity of hybrid peptide P18 and its mechanism of action.
Doi : https://doi.org/10.1007/s10529-009-0175-2
Abstract : The anticancer properties and mechanism of action of a hybrid peptide -P18 were investigated. It had significant cytotoxic activity against human melanoma cells and low toxicity to normal NIH-3T3 cells. It also induced cell death via necrosis rather than classical apoptosis. The peptide targets the cells membrane, causing a sustained depolarization of transmembrane potential resulting in the cells swelling and bursting, thereby triggering cytolysis. P18 peptide initially binds to the melanoma cell membrane via electrostatic interaction, causing the cell membrane to rupture. The effect may be mediated by the amphiphilic alpha-helical structure of P18 peptide, coupled with changes in ion channels and an increase in plasma membrane permeability that eventually leads to melanoma cell death.