Peptide name : Peptide Glycyrrhetinic Acid Based Derivatives (compound 5)

Source/Organism : Not found

Linear/Cyclic : Linear

Chirality : L

Peptide length: 4

C-terminal modification: Linear

N-terminal modification : Not found

Non-natural peptide information: None

Assay type : Not specified

Assay time : Not found

Activity : IC50 : 5.0 ± 0.3 µg/mL

Cell line : MCF-7

Cancer type : Cervical cancer

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 246.2205 Dalton

Aliphatic index : 0

Instability index : 100.05

Hydrophobicity (GRAVY) : -0.4

Isoelectric point : 5.525

Charge (pH 7) : -0.2399

Aromaticity : 0

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : infinite

hydrophobic moment : -0.038

Missing amino acid : W,T,P,I,M,E,K,F,D,N,C,R,H,Q,S,Y,L,A,V

Most occurring amino acid : G

Most occurring amino acid frequency : 4

Least occurring amino acid : G

Least occurring amino acid frequency : 4

Secondary structure fraction (Helix, Turn, Sheet): (0, 1, 0)

SMILES Notation: NCC(=O)NCC(=O)NCC(=O)NCC(=O)O

1 : Xiong C, et al. Induction of Apoptosis in HeLa Cells by a Novel Peptide from Fruiting Bodies of Morchella importuna via the Mitochondrial Apoptotic Pathway. Evid Based Complement Alternat Med. 2021; 2021:5563367. doi: 10.1155/2021/5563367

Paper title : Induction of Apoptosis in HeLa Cells by a Novel Peptide from Fruiting Bodies of Morchella importuna via the Mitochondrial Apoptotic Pathway.

Doi : https://doi.org/10.1155/2021/5563367

Abstract : Morels (Morchella spp.) are a genus of edible fungi with important economic and medicinal value. In this study, a novel peptide (MIPP) was extracted from the fruiting bodies of Morchella importuna using gel filtration chromatography. Structural analysis showed that the molecular mass of MIPP is 831 Da, and it has a simple amino acid sequence: Ser-Leu-Ser-Leu-Ser-Val-Ala-Arg. To explore the antitumor activity of MIPP, the effect of MIPP on HeLa cell apoptosis and the underlying preventative mechanisms were investigated. Results showed that MIPP reduced the viability of HeLa cells in a concentration-dependent manner. TUNEL analysis and flow cytometric examination showed that MIPP decreased cell proliferation via a mitochondrial-dependent pathway, as manifested by downregulation of Bcl-2/Bax, promotion of the movement of cytochrome C from the mitochondria to the cytoplasm, and triggering of caspase-9 and caspase-3. Therefore, MIPP may be a promising tumor-preventive agent, especially in human cervical cancer.