Peptide name : Phthiocerol/phthiodiolone dimycocerosyl transferase (EC 2.3.1.282) (Acyltransferase PapA5) (Phthiocerol/phthiodiolone O-acyltransferase) (Polyketide synthase-associated protein A5)

Source/Organism : Aeromonads

Linear/Cyclic : Not found

Chirality : Not found

Peptide length: 390

C-terminal modification: Not found

N-terminal modification : Free

Non-natural peptide information: None

Assay type : Toxicity assay, Cell nucleus fragmentation assay(cnf)

Assay time : 5days

Activity : MIC : 50 ng/ml

Cell line : L929

Cancer type : Not specified

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 43235.186 Dalton

Aliphatic index : 0.969

Instability index : 41.7621

Hydrophobicity (GRAVY) : -0.144

Isoelectric point : 10.034

Charge (pH 7) : 9.6091

Aromaticity : 0.061

Molar extinction coefficient (cysteine, cystine): (27960, 28085)

Hydrophobic/hydrophilic ratio : 1.17877095

hydrophobic moment : 0.0258

Missing amino acid : None

Most occurring amino acid : L

Most occurring amino acid frequency : 57

Least occurring amino acid : C

Least occurring amino acid frequency : 2

Secondary structure fraction (Helix, Turn, Sheet): (0.3, 0.2, 0.3)

SMILES Notation: 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1 : Sandmann A, et al. Identification and analysis of the core biosynthetic machinery of tubulysin, a potent cytotoxin with potential anticancer activity. Chem Biol. 2004; 11:1071-9. doi: 10.1016/j.chembiol.2004.05.014

Paper title : Identification and analysis of the core biosynthetic machinery of tubulysin, a potent cytotoxin with potential anticancer activity.

Doi : https://doi.org/10.1016/j.chembiol.2004.05.014

Abstract : Myxobacteria are well known for their biosynthetic potential, especially for the production of cytotoxic compounds with potential anticancer activities. The tubulysins are currently in preclinical development. They are produced in very low quantities, and genetic manipulation of producing strains has never been accomplished. We report the development of a mariner-based transposon mutagenesis system for Angiococcus disciformis An d48. Extracts from a library of 1200 mutants were analyzed for the presence of tubulysin by a microscopic cell nucleus fragmentation bioassay. The transposition sites of four tubulysin-negative mutants were identified by vector recovery, which led to the identification and the sequencing of the corresponding core biosynthetic gene locus. Sequence analysis of more than 80,000 bp reveals an unusual multimodular hybrid polyketide synthase/peptide synthetase assembly line with a variety of unprecedented features.