Peptide name : Phylloseptin-PBa1

Source/Organism : Burmeister's leaf frog, Brazil, South America

Linear/Cyclic : Linear

Chirality : Not found

Peptide length: 19

C-terminal modification: Linear

N-terminal modification : Amidation

Non-natural peptide information: None

Assay type : MTT cell viability assay

Assay time : 72h

Activity : IC50 : approx. 0.29 µM

Cell line : MCF-7

Cancer type : Breast cancer

Other activity : Anti-microbial activity; Hemolytic activity

Amino acid composition bar chart :

Molecular mass : 2027.4079 Dalton

Aliphatic index : 1.489

Instability index : 17.1895

Hydrophobicity (GRAVY) : 1.2263

Isoelectric point : 8.7572

Charge (pH 7) : 0.8463

Aromaticity : 0.105

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 2.16666666

hydrophobic moment : 1.7032

Missing amino acid : C,R,W,Q,T,M,E,D,Y

Most occurring amino acid : L

Most occurring amino acid frequency : 3

Least occurring amino acid : P

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0.3, 0.3, 0.4)

SMILES Notation: CC[C@H](C)[C@H](NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)Cc1ccccc1)[C@@H](C)CC)[C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](Cc1ccccc1)C(=O)O)C(C)C

1 : Wu Y, et al. Phylloseptin-PBa1, -PBa2, -PBa3: Three novel antimicrobial peptides from the skin secretion of Burmeister's leaf frog (Phyllomedusa burmeisteri). Biochem Biophys Res Commun. 2019; 509:664-673. doi: 10.1016/j.bbrc.2018.12.156

Paper title : Phylloseptin-PBa1, -PBa2, -PBa3: Three novel antimicrobial peptides from the skin secretion of Burmeister's leaf frog (Phyllomedusa burmeisteri).

Doi : https://doi.org/10.1016/j.bbrc.2018.12.156

Abstract : Skin secretions are known as a highly-complex mixture of abundant and diverse bioactive molecules and its study has attracted increasing attention over recent years. Phylloseptin is a unique family of antimicrobial peptides which have been only isolated from frogs of the Phyllomedusinae subfamily. Here, three novel peptide precursors were successfully cloned from a cDNA library, which was constructed from the skin secretion of Phyllomedusa burmeisteri, as pair of primers (one nested universal primer and a designed degenerate sense primer) were employed for "shotgun" cloning. The encoded mature peptides were validated by MS/MS sequencing, and subsequently termed as Phylloseptin-PBa1, -PBa2 and -PBa3. Phylloseptin-PBa1 and -PBa2 were demonstrated to possess potent antimicrobial activities against Gram-positive bacteria and yeast, as well as broad-spectrum anticancer activities, while they possess varying haemolytic activity at the effective concentration. In contrast, Phylloseptin-PBa3 was found to exhibit a strong haemolytic activity even though it was only found to possess a weak antimicrobial activity and inconspicuous anticancer activity.