Peptide name : Piscidin-4

Source/Organism : Nile tilapia

Linear/Cyclic : Linear

Chirality : D

Peptide length: 25

C-terminal modification: Linear

N-terminal modification : Not found

Non-natural peptide information: None

Assay type : Adenosine triphosphate (ATP) assay

Assay time : 3–24h

Activity : IC50 : 3.769 – 14.17 µM

Cell line : NCI-H661

Cancer type : Non-small cell Lung cancer (NSCLC)

Other activity : Anti-microbial activity

Amino acid composition bar chart :

Molecular mass : 2981.5575 Dalton

Aliphatic index : 1.172

Instability index : 130.236

Hydrophobicity (GRAVY) : -0.128

Isoelectric point : 12

Charge (pH 7) : 7.0206

Aromaticity : 0.08

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 1.27272727

hydrophobic moment : 0.8206

Missing amino acid : C,W,Q,T,P,M,E,D,Y,N,V

Most occurring amino acid : R

Most occurring amino acid frequency : 6

Least occurring amino acid : S

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0.2, 0.1, 0.3)

SMILES Notation: CC[C@H](C)[C@H](NC(=O)[C@H](C)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@@H](NC(=O)[C@@H](N)Cc1ccccc1)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)CC)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)O)[C@@H](C)CC

1 : Ting CH and Chen JY. Nile Tilapia Derived TP4 Shows Broad Cytotoxicity Toward to Non-Small-Cell Lung Cancer Cells. Mar Drugs. 2018; 16:(unknown pages). doi: 10.3390/md16120506

Paper title : Nile Tilapia Derived TP4 Shows Broad Cytotoxicity Toward to Non-Small-Cell Lung Cancer Cells.

Doi : https://doi.org/10.3390/md16120506

Abstract : Non-small cell lung cancer (NSCLC) is among the leading causes of human mortality due to a lack of effective treatments. Conventional chemotherapies affect healthy cells and cause multidrug resistance, while tumors may eventually develop resistance to less-toxic targeted therapies. Thus, the need to develop novel therapies for NSCLC is urgent. Here, we show that Nile tilapia-derived Tilapia piscidin (TP) 4 is cytotoxic to a panel of NSCLC cells with different genetic profiles. We observed that TP4 triggers NSCLC cell death through the necrosis and combining TP4 with potent Epidermal growth factor receptor (EGFR)- tyrosine kinase inhibitors (TKI)s, Erlotinib, and Gefitinib, improved drug responses in EGFR-mutated NSCLC cells, but not in EGFR-wild-type NSCLC cells. This work provides novel insights into potential NSCLC treatments, which may utilize antimicrobial peptide TP4 as monotherapy or in combination with EGFR-TKIs.