Peptide name : Putative nonribosomal peptide synthetase P4-B4-NRPS

Source/Organism : Gram-negative bacillus

Linear/Cyclic : Cyclic

Chirality : Not found

Peptide length: 1204

C-terminal modification: Cyclic

N-terminal modification : Not found

Non-natural peptide information: None

Assay type : GFP assay

Assay time : Not found

Activity : Not found

Cell line : NIH 3T3

Cancer type : Cutaneous T-cell lymphoma

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 130142.066 Dalton

Aliphatic index : 0.955

Instability index : 40.0593

Hydrophobicity (GRAVY) : -0.018

Isoelectric point : 4.9023

Charge (pH 7) : -42.8052

Aromaticity : 0.081

Molar extinction coefficient (cysteine, cystine): (236170, 236545)

Hydrophobic/hydrophilic ratio : 1.51356993

hydrophobic moment : -0.051

Missing amino acid : None

Most occurring amino acid : A

Most occurring amino acid frequency : 182

Least occurring amino acid : C

Least occurring amino acid frequency : 6

Secondary structure fraction (Helix, Turn, Sheet): (0.3, 0.2, 0.3)

SMILES Notation: 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1 : Cheng YQ, et al. Characterization of a gene cluster responsible for the biosynthesis of anticancer agent FK228 in Chromobacterium violaceum No. 968. Appl Environ Microbiol. 2007; 73:3460-9. doi: 10.1128/AEM.01751-06

Paper title : Characterization of a gene cluster responsible for the biosynthesis of anticancer agent FK228 in Chromobacterium violaceum No. 968.

Doi : https://doi.org/10.1128/AEM.01751-06

Abstract : A gene cluster responsible for the biosynthesis of anticancer agent FK228 has been identified, cloned, and partially characterized in Chromobacterium violaceum no. 968. First, a genome-scanning approach was applied to identify three distinctive C. violaceum no. 968 genomic DNA clones that code for portions of nonribosomal peptide synthetase and polyketide synthase. Next, a gene replacement system developed originally for Pseudomonas aeruginosa was adapted to inactivate the genomic DNA-associated candidate natural product biosynthetic genes in vivo with high efficiency. Inactivation of a nonribosomal peptide synthetase-encoding gene completely abolished FK228 production in mutant strains. Subsequently, the entire FK228 biosynthetic gene cluster was cloned and sequenced. This gene cluster is predicted to encompass a 36.4-kb DNA region that includes 14 genes. The products of nine biosynthetic genes are proposed to constitute an unusual hybrid nonribosomal peptide synthetase-polyketide synthase-nonribosomal peptide synthetase assembly line including accessory activities for the biosynthesis of FK228. In particular, a putative flavin adenine dinucleotide-dependent pyridine nucleotide-disulfide oxidoreductase is proposed to catalyze disulfide bond formation between two sulfhydryl groups of cysteine residues as the final step in FK228 biosynthesis. Acquisition of the FK228 biosynthetic gene cluster and acclimation of an efficient genetic system should enable genetic engineering of the FK228 biosynthetic pathway in C. violaceum no. 968 for the generation of structural analogs as anticancer drug candidates.