Peptide name : Saha-CATH5

Source/Organism : Tasmanian devil

Linear/Cyclic : Not found

Chirality : Not found

Peptide length: 20

C-terminal modification: Not found

N-terminal modification : Free

Non-natural peptide information: None

Assay type : Not specified

Assay time : Not found

Activity : MIC : 500 μg/mL

Cell line : A549

Cancer type : Lung cancer

Other activity : Anti-bacterial activity

Amino acid composition bar chart :

Molecular mass : 2301.912 Dalton

Aliphatic index : 1.755

Instability index : 70.595

Hydrophobicity (GRAVY) : 0.255

Isoelectric point : 12

Charge (pH 7) : 6.7581

Aromaticity : 0

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 1.85714285

hydrophobic moment : 0.7245

Missing amino acid : C,W,H,Q,T,P,M,E,F,S,D,Y,N,A,V

Most occurring amino acid : R

Most occurring amino acid frequency : 5

Least occurring amino acid : K

Least occurring amino acid frequency : 2

Secondary structure fraction (Helix, Turn, Sheet): (0.3, 0.2, 0.4)

SMILES Notation: CC[C@H](C)[C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H](N)CCCCN)[C@@H](C)CC)[C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)O)[C@@H](C)CC

1 : Peel E, et al. Cathelicidins in the Tasmanian devil (Sarcophilus harrisii). Sci Rep. 2016; 6:35019. doi: 10.1038/srep35019

Paper title : Cathelicidins in the Tasmanian devil (Sarcophilus harrisii).

Doi : https://doi.org/10.1038/srep35019

Abstract : Tasmanian devil joeys, like other marsupials, are born at a very early stage of development, prior to the development of their adaptive immune system, yet survive in a pathogen-laden pouch and burrow. Antimicrobial peptides, called cathelicidins, which provide innate immune protection during early life, are expressed in the pouch lining, skin and milk of devil dams. These peptides are active against pathogens identified in the pouch microbiome. Of the six characterised cathelicidins, Saha-CATH5 and 6 have broad-spectrum antibacterial activity and are capable of killing problematic human pathogens including methicillin-resistant S. aureus and vancomycin-resistant E. faecalis, while Saha-CATH3 is active against fungi. Saha-CATH5 and 6 were toxic to human A549 cells at 500 μg/mL, which is over seven times the concentration required to kill pathogens. The remaining devil cathelicidins were not active against tested bacterial or fungal strains, but are widely expressed throughout the body, such as in immune tissues, in digestive, respiratory and reproductive tracts, and in the milk and pouch, which indicates that they are likely also important components of the devil immune system. Our results suggest cathelicidins play a role in protecting naive young during pouch life by passive immune transfer in the milk and may modulate pouch microbe populations to reduce potential pathogens.