dbacp06035
General Description
Peptide name : Scolopin-2
Source/Organism : Chinese red-headed centipede
Linear/Cyclic : Not found
Chirality : Not found
Sequence Information
Sequence : ILKKFMLHRGTKVYKMRTLSKRSH
Peptide length: 24
C-terminal modification: Not found
N-terminal modification : Amidation
Non-natural peptide information: None
Activity Information
Assay type : MTT assay
Assay time : 4h
Activity : IC50 : 35 μM
Cell line : Hela
Cancer type : Cervical cancer
Other activity : Anti-microbial activity
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 2959.6266 Dalton
Aliphatic index : 0.770
Instability index : 42.65
Hydrophobicity (GRAVY) : -0.725
Isoelectric point : 11.763
Charge (pH 7) : 7.9284
Aromaticity : 0.083
Molar extinction coefficient (cysteine, cystine): (1490, 1490)
Hydrophobic/hydrophilic ratio : 0.6
hydrophobic moment : 0.2321
Missing amino acid : C,W,Q,P,E,D,N,A
Most occurring amino acid : K
Most occurring amino acid frequency : 5
Least occurring amino acid : I
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0.4, 0.1, 0.3)
SMILES Notation: CC[C@H](C)[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)O)[C@@H](C)O)C(C)C)[C@@H](C)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | HHHHHHHHTTTHHHEHHHHHHTTT |
| Chou-Fasman (CF) | HHHHHHHCCEEEECCEEECCCCCC |
| Neural Network (NN) | HHHHHHHHHCCHHHHHHHHCCCCC |
| Joint/Consensus | HHHHHHHHCCCCCCCHHHHCCCCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
CancerPPD : Not available
ApIAPDB : Not available
CancerPPD2 ID : Not available
Reference
1 : Yan W, et al. Amidated Scolopin-2 inhibits proliferation and induces apoptosis of Hela cells in vitro and in vivo. Biotechnol Appl Biochem. 2018; 65:672-679. doi: 10.1002/bab.1661
Literature
Paper title : Amidated Scolopin-2 inhibits proliferation and induces apoptosis of Hela cells in vitro and in vivo.
Doi : https://doi.org/10.1002/bab.1661
Abstract : This study aimed to investigate the effect of Scolopin-2, a cationic antimicrobial peptide from centipede venoms, and amidated Scolopin-2 on Hela cell viability in vitro and in vivo. The cellular proliferation was investigated with the MTT assay. Confocal laser scanning, flow cytometry, and Western blot analysis were employed to localize Scolopin-2-NH<sub>2</sub> in Hela cells and to study the caused cells apoptosis. We subcutaneously injected Hela cells into BALB/c nude mice and studied if Scolopin-2-NH<sub>2</sub> suppressed tumor growth in the mice. Scolopin-2-NH<sub>2</sub> inhibited Hela proliferation in vitro in a dose-dependent manner with an IC<sub>50</sub> of 35 μM. In addition, Scolopin-2-NH<sub>2</sub> combined with mitochondria and regulated caspase-related apoptosis pathways in Hela cells. Scolopin-2-NH<sub>2</sub> significantly suppressed tumor growth in the tumor-bearing mice without side effects, such as weight loss or abnormal changes in tissues, including liver, spleen, kidney, and lung. These results indicate Scolopin-2-NH<sub>2</sub> may be a good therapeutic candidate for the treatment of Hela cervical cancer.