Peptide name : Tachyplesin I

Source/Organism : Hemocytes, Southeast Asia, Japanese horseshoe crab

Linear/Cyclic : Not found

Chirality : Not found

Peptide length: 17

C-terminal modification: Not found

N-terminal modification : Free

Non-natural peptide information: None

Assay type : Luciferase reporter assay, TUNEL assay

Assay time : 24h

Activity : Not found

Cell line : T167

Cancer type : Squamous cell oral carcinoma

Other activity : Anti- microbial activity

Amino acid composition bar chart :

Molecular mass : 2268.7577 Dalton

Aliphatic index : 0.4

Instability index : 13.2235

Hydrophobicity (GRAVY) : -0.517

Isoelectric point : 9.931

Charge (pH 7) : 5.7175

Aromaticity : 0.235

Molar extinction coefficient (cysteine, cystine): (8480, 8730)

Hydrophobic/hydrophilic ratio : 1.125

hydrophobic moment : -0.291

Missing amino acid : H,Q,T,P,M,E,S,D,L,N,A

Most occurring amino acid : R

Most occurring amino acid frequency : 5

Least occurring amino acid : K

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0.0, 0.0, 0.3)

SMILES Notation: CC[C@H](C)[C@H](NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CS)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@@H](N)CCCCN)C(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(=N)N)C(=O)O

1 : Nakamura T, et al. Tachyplesin, a class of antimicrobial peptide from the hemocytes of the horseshoe crab (Tachypleus tridentatus). Isolation and chemical structure. J Biol Chem. 1988; 263:16709-13.

Paper title : Tachyplesin, a class of antimicrobial peptide from the hemocytes of the horseshoe crab (Tachypleus tridentatus). Isolation and chemical structure.

Doi : https://doi.org/Not available

Abstract : A cationic peptide, designated tachyplesin, was isolated from acid extracts of horseshoe crab (Tachypleus tridentatus) hemocyte debris. It consists of 17 residues and the structure determined by Edman degradation is: (formula; see text) The carboxyl-terminal end of this peptide was identified as arginine alpha-amide, and the whole sequence including the alpha-amide was also confirmed by fast atom bombardment mass spectrometry, indicating a mass value of 2263. Tachyplesin inhibits growth of both Gram-negative and -positive bacteria at low concentrations and formed a complex with bacterial lipopolysaccharide. Tachyplesin seems likely to act as antimicrobial peptide for self-defense in the horseshoe crab against invading microorganisms.