dbacp06195
General Description
Peptide name : Tat
Source/Organism : HIV-Tat (49-57)
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : RKKRRQRRR
Peptide length: 9
C-terminal modification: Linear
N-terminal modification : Free
Non-natural peptide information: None
Activity Information
Assay type : MTT/MTS assay
Assay time : 24h
Activity : ~10% cytotoxicity at 100 µM
Cell line : HCT-116
Cancer type : Colon cancer
Other activity : Not found
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 1339.6033 Dalton
Aliphatic index : 0
Instability index : 257.444
Hydrophobicity (GRAVY) : -4.255
Isoelectric point : 12
Charge (pH 7) : 7.758
Aromaticity : 0
Molar extinction coefficient (cysteine, cystine): (0, 0)
Hydrophobic/hydrophilic ratio : 0
hydrophobic moment : -0.659
Missing amino acid : W,T,P,I,M,E,F,D,N,G,C,H,S,Y,L,A,V
Most occurring amino acid : R
Most occurring amino acid frequency : 6
Least occurring amino acid : Q
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0.2, 0, 0)
SMILES Notation: N=C(N)NCCC[C@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCCNC(=N)N)C(=O)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | HHHHHHHHT |
| Chou-Fasman (CF) | HHHHHHCCC |
| Neural Network (NN) | CCCCCCCHH |
| Joint/Consensus | HHHHHHCCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
Reference
1 : Lou H, et al. A novel peptide from alpha5 helix of Asterina pectinifera cyclin B conjugated to HIV-Tat(49-57) with cytotoxic and apoptotic effects against human cancer cells. Bioorg Med Chem Lett. 2008; 18:4633-7. doi: 10.1016/j.bmcl.2008.07.017
Literature
Paper title : A novel peptide from alpha5 helix of Asterina pectinifera cyclin B conjugated to HIV-Tat(49-57) with cytotoxic and apoptotic effects against human cancer cells.
Doi : https://doi.org/10.1016/j.bmcl.2008.07.017
Abstract : A series of novel peptides from various motifs of Asterina pectinifera cyclin B and their derivatives conjugated to HIV-Tat(49-57) were designed and synthesized. Their bioactivities on two human cancer cell lines were determined. Among them, Tat-a5 (KAQIRAMECNILGRKKRRQRRR) exhibited significant cytotoxic effects on cancer cell lines EC-9706 and HCT-116. Tat-a5 could arrest cancer cells at G(2)/M phase and make them apoptotic. Our results suggested that Tat-a5 could be a novel leading peptide with anticancer activity.