dbacp06307
General Description
Peptide name : TLS peptide
Source/Organism : Not found
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : TLSGAFELSRDK
Peptide length: 12
C-terminal modification: Linear
N-terminal modification : Not found
Non-natural peptide information: None
Activity Information
Assay type : Not specified
Assay time : Not found
Activity : Not found
Cell line : SKOV3
Cancer type : Ovarian cancer
Other activity : Not found
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 1323.4514 Dalton
Aliphatic index : 0.733
Instability index : 11.0667
Hydrophobicity (GRAVY) : -0.491
Isoelectric point : 5.735
Charge (pH 7) : -0.5989
Aromaticity : 0.083
Molar extinction coefficient (cysteine, cystine): (0, 0)
Hydrophobic/hydrophilic ratio : 0.71428571
hydrophobic moment : 0.3196
Missing amino acid : C,W,H,Q,P,M,I,Y,N,V
Most occurring amino acid : L
Most occurring amino acid frequency : 2
Least occurring amino acid : T
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0.4, 0.3, 0.3)
SMILES Notation: CC(C)C[C@H](NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](C)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)[C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCCCN)C(=O)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | EETTHHHHHHTT |
| Chou-Fasman (CF) | CCCHHHHHCCCC |
| Neural Network (NN) | CCCCCHHHCCCC |
| Joint/Consensus | CCCCHHHHCCCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
CancerPPD : Not available
ApIAPDB : Not available
CancerPPD2 ID : Not available
Reference
1 : Ma C, et al. A specific cell-penetrating peptide induces apoptosis in SKOV3 cells by down-regulation of Bcl-2. Biotechnol Lett. 2013; 35:1791-7. doi: 10.1007/s10529-013-1263-x
Literature
Paper title : A specific cell-penetrating peptide induces apoptosis in SKOV3 cells by down-regulation of Bcl-2.
Doi : https://doi.org/10.1007/s10529-013-1263-x
Abstract : Peptides are emerging as pharmaceutical agents in cancer therapy. The peptide, TLSGAFELSRDK (TLS) is a targeting ligand that can specifically triggers cellular uptake by binding to SKOV3 cells. Cell surface proteins and the C-terminal basic residues of the TLS are required for effective cell penetration, and the uptake process is energy-dependent. It inhibited the proliferation of SKOV3 cells and induced early-stage apoptosis by down-regulation of Bcl-2 expression mediated through a caspase-dependent pathway. The synergistic anti-proliferative effects of the peptide TLS and doxorubicin on SKOV3 cells were further investigated. Taken together, TLS, acting as a combination of a targeted ligand and a therapeutic agent, was a promising candidate for the development of peptide-based therapies in ovarian cancer.