dbacp06410
General Description
Peptide name : Tyroserleutide
Source/Organism : Synthetic Peptide
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : YSL
Peptide length: 3
C-terminal modification: Linear
N-terminal modification : Free
Non-natural peptide information: None
Activity Information
Assay type : MTT/MTS assay
Assay time : 96h
Activity : 33.8% Cytotoxic at 10 µg/ml
Cell line : BEL-7402
Cancer type : Liver cancer
Other activity : Not found
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 381.4234 Dalton
Aliphatic index : 1.3
Instability index : 6.6667
Hydrophobicity (GRAVY) : 0.5667
Isoelectric point : 5.5243
Charge (pH 7) : -0.2409
Aromaticity : 0.333
Molar extinction coefficient (cysteine, cystine): (1490, 1490)
Hydrophobic/hydrophilic ratio : 0.5
hydrophobic moment : -1.577
Missing amino acid : W,T,P,I,M,E,K,F,D,N,G,C,R,H,Q,A,V
Most occurring amino acid : Y
Most occurring amino acid frequency : 1
Least occurring amino acid : Y
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0.3, 0.3, 0.6)
SMILES Notation: CC(C)C[C@H](NC(=O)[C@H](CO)NC(=O)[C@@H](N)Cc1ccc(O)cc1)C(=O)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | EEE |
| Chou-Fasman (CF) | CCC |
| Neural Network (NN) | CCC |
| Joint/Consensus | CCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
Reference
1 : Yao Z, et al. Tripeptide tyroserleutide enhances the antitumor effects of macrophages and stimulates macrophage secretion of IL-1beta, TNF-alpha, and NO in vitro. Cancer Immunol Immunother. 2006; 55:56-60. doi: 10.1007/s00262-005-0024-7
Literature
Paper title : Tripeptide tyroserleutide enhances the antitumor effects of macrophages and stimulates macrophage secretion of IL-1beta, TNF-alpha, and NO in vitro.
Doi : https://doi.org/10.1007/s00262-005-0024-7
Abstract : Tyroserleutide (YSL) is a type of active, low molecular weight polypeptide, comprised of three amino acids, which has antitumor effects. YSL has various advantages over the other bioactive peptides such as its low molecular weight, simple construction, nonimmunogenicity, specificity, few side effects, and ease of synthesis. However, the biological activities contributing to it's antitumor effects are not yet known. We studied the effects of YSL on the in vitro cytotoxic activity of BALB/c mice peritoneal macrophages (PEMphi) against the target tumor cell lines BEL-7402 and B16-F10. We also measured the concentrations of interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha, and nitric oxide (NO) produced by YSL-activated Mphi, and we determined the concentrations of IL-1beta and NO secreted by YSL-activated murine macrophage RAW264.7 cells. YSL activated Mphi in vitro, inhibited BEL-7402 proliferation, enhanced PEMphi antitumor effects, and stimulated IL-1beta, TNF-alpha, and NO production by RAW264.7 cells. These data suggest that YSL activates the monocyte-macrophage system, which enhances Mphi antitumor effects against BEL-7402 and B16-F10 cells and stimulates the secretion by Mphi of cytotoxic effectors such as IL-1beta, TNF-alpha, and NO.