Peptide name : Tyrosine-protein kinase Lck-486 peptide

Source/Organism : Tyrosine-protein kiNot foundse Lck-486 peptide

Linear/Cyclic : Not found

Chirality : Not found

Peptide length: 9

C-terminal modification: Not found

N-terminal modification : Not found

Non-natural peptide information: None

Assay type : ELISPOT assay

Assay time : 3days

Activity : Not found

Cell line : Colon26

Cancer type : Murine rectal cancer

Other activity : Not found

Amino acid composition bar chart :

Molecular mass : 1113.2628 Dalton

Aliphatic index : 1.188

Instability index : 106.533

Hydrophobicity (GRAVY) : 0.4222

Isoelectric point : 5.4983

Charge (pH 7) : -0.6017

Aromaticity : 0.222

Molar extinction coefficient (cysteine, cystine): (1490, 1490)

Hydrophobic/hydrophilic ratio : 0.8

hydrophobic moment : 1.4242

Missing amino acid : C,W,H,Q,P,M,I,E,K,N,A,G

Most occurring amino acid : L

Most occurring amino acid frequency : 2

Least occurring amino acid : T

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (0.2, 0.2, 0.6)

SMILES Notation: CC(C)C[C@H](NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@@H](N)[C@@H](C)O)C(C)C)C(=O)O

1 : Matsueda S, et al. Antitumor activity of antibody against cytotoxic T lymphocyte epitope peptide of lymphocyte-specific protein tyrosine kinase. Cancer Sci. 2018; 109:611-617. doi: 10.1111/cas.13522

Paper title : Antitumor activity of antibody against cytotoxic T lymphocyte epitope peptide of lymphocyte-specific protein tyrosine kinase.

Doi : https://doi.org/10.1111/cas.13522

Abstract : Although humoral responses against CTL epitope peptides from lymphocyte-specific protein tyrosine kinase (Lck) antigen have been observed in the majority of healthy donors and cancer patients, the biological activity of the antibody has not been determined. We investigated the biological activity of mAb against CTL epitope peptide of Lck antigen at positions 486-494 (anti-Lck-486 mAb). This mAb induced dendritic cell maturation from murine bone marrow cells by the immune complex form in vitro, and inhibited tumor growth in association with a suppression of tumor-infiltrating T cells, including T regulatory cells in a murine model using female BALB/cCrlCrlj mice (H-2Kd ). More potent tumor inhibition was observed when this mAb was given prior to peptide vaccination. These results may help to unveil the biological activity of anti-Lck peptide antibodies against CTL epitope peptides.