Peptide name : Z44

Source/Organism : African clawed frog

Linear/Cyclic : Linear

Chirality : L

Peptide length: 24

C-terminal modification: Linear

N-terminal modification : Amidation

Non-natural peptide information: None

Assay type : Trypan blue assay

Assay time : 72h

Activity : IC50 : 80 µg/ml

Cell line : MLA

Cancer type : Leukemia cancer

Other activity : Anti-microbial activity

Amino acid composition bar chart :

Molecular mass : 2414.9259 Dalton

Aliphatic index : 1.225

Instability index : 9.5833

Hydrophobicity (GRAVY) : 0.225

Isoelectric point : 10.699

Charge (pH 7) : 5.7899

Aromaticity : 0

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 1

hydrophobic moment : -0.767

Missing amino acid : W,T,P,I,M,E,F,D,N,G,C,R,H,Q,Y,V

Most occurring amino acid : A

Most occurring amino acid frequency : 6

Least occurring amino acid : A

Least occurring amino acid frequency : 6

Secondary structure fraction (Helix, Turn, Sheet): (0.7, 0.2, 0.2)

SMILES Notation: CC(C)C[C@H](NC(=O)[C@H](C)N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)O

1 : Cruciani RA, et al. Antibiotic magainins exert cytolytic activity against transformed cell lines through channel formation. Proc Natl Acad Sci U S A. 1991; 88:3792-6. doi: 10.1073/pnas.88.9.3792

2 : Huang CM, et al. Magainin analogs effective against pathogenic protozoa. Antimicrob Agents Chemother. 1990; 34:1824-6. doi: 10.1128/AAC.34.9.1824

Paper title : Antibiotic magainins exert cytolytic activity against transformed cell lines through channel formation.

Doi : https://doi.org/10.1073/pnas.88.9.3792

Abstract : Magainins are an ionophoric class of vertebrate peptides with antibiotic activity against various microorganisms. Here we show that magainin 2 and synthetic analogues can rapidly and irreversibly lyse hematopoietic tumor and solid tumor target cells with a relative cytotoxic potency that parallels their antibacterial efficacy and at concentrations that are relatively nontoxic to well-differentiated cells. The cytotoxicity is prevented by cell depolarization. Magainins represent a natural cytolytic agent in vertebrates and may provide another therapeutic strategy for certain tumors.

Paper title : Magainin analogs effective against pathogenic protozoa.

Doi : https://doi.org/10.1128/AAC.34.9.1824

Abstract : The in vitro activities of magainin analogs against Blastocystis hominis, Entamoeba histolytica, and Trypanosoma cruzi were assessed by protozoan morphological integrity and motility. The antiprotozoan activities in descending order were magainin B greater than G greater than H, the same order as the alpha-helix contents of the analogs. Magainin B and G were effective against B. hominis, T. cruzi, and E. histolytica.