Peptide name : NT-9

Source/Organism : Synthetic analogue of Neurotensin

Linear/Cyclic : Linear

Chirality : L

Peptide length: 6

C-terminal modification: Linear

N-terminal modification : Free

Non-natural peptide information:

Assay type : MTS assay

Assay time : 24-h

Activity : Not Available

Cell line : HCT-116

Cancer type : Colorectal Cancer

Other activity : Anticancer

Amino acid composition bar chart :

Molecular mass : 816.9903 Dalton

Aliphatic index : 1.3

Instability index : 168

Hydrophobicity (GRAVY) : -0.6

Isoelectric point : 10.835

Charge (pH 7) : 1.7591

Aromaticity : 16.66

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 1

hydrophobic moment : -0.388

Missing amino acid : A,C,D,E,F,G,H,K,M,N,Q,S,T,V,W

Most occurring amino acid : R

Most occurring amino acid frequency : 2

Least occurring amino acid : P

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (16., 16., 50.)

SMILES Notation: CC[C@H](C)[C@H](NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H](N)CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)O

1 : Szaryńska M, et al. Bradykinin and Neurotensin Analogues as Potential Compounds in Colon Cancer Therapy. Int J Mol Sci. 2023; 24:(unknown pages). doi: 10.3390/ijms24119644

Paper title : Bradykinin and Neurotensin Analogues as Potential Compounds in Colon Cancer Therapy.

Doi : https://doi.org/10.3390/ijms24119644

Abstract : Colorectal cancer (CRC) is one of the most lethal malignancies worldwide, so the attempts to find novel therapeutic approaches are necessary. The aim of our study was to analyze how chemical modifications influence physical, chemical, and biological properties of the two peptides, namely, bradykinin (BK) and neurotensin (NT). For this purpose, we used fourteen modified peptides, and their anti-cancers features were analyzed on the HCT116 CRC cell line. Our results confirmed that the spherical mode of a CRC cell line culture better reflects the natural tumour microenvironment. We observed that the size of the colonospheres was markedly reduced following treatment with some BK and NT analogues. The proportion of CD133+ cancer stem cells (CSCs) in colonospheres decreased following incubation with the aforementioned peptides. In our research, we found two groups of these peptides. The first group influenced all the analyzed cellular features, while the second seemed to include the most promising peptides that lowered the count of CD133+ CSCs with parallel substantial reduction in CRC cells viability. These analogues need further analysis to uncover their overall anti-cancer potential.