dbacp06942
General Description
Peptide name : HTDT-6-2-3-2
Source/Organism : Enteromorpha prolifera
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : GPLGAGP
Peptide length: 7
C-terminal modification: Linear
N-terminal modification : Free
Non-natural peptide information:
Activity Information
Assay type : CCK-8 assay
Assay time : 48-h
Activity : IC50 = 0.3686 ± 0.0935 mg/mL
Cell line : NCI-H-460
Cancer type : Lung Cancer
Other activity : Anticancer
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 567.6351 Dalton
Aliphatic index : 0.7
Instability index : -3.5571
Hydrophobicity (GRAVY) : 0.1714
Isoelectric point : 5.525
Charge (pH 7) : -0.2399
Aromaticity : 0
Molar extinction coefficient (cysteine, cystine): (0, 0)
Hydrophobic/hydrophilic ratio : infinite
hydrophobic moment : -0.201
Missing amino acid : C,D,E,F,H,I,K,M,N,Q,R,S,T,V,W,Y
Most occurring amino acid : G
Most occurring amino acid frequency : 3
Least occurring amino acid : L
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (28., 71., 14.)
SMILES Notation: CC(C)C[C@H](NC(=O)[C@@H]1CCCN1C(=O)CN)C(=O)NCC(=O)N[C@@H](C)C(=O)NCC(=O)N1CCC[C@H]1C(=O)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | CCCTCCC |
| Chou-Fasman (CF) | CCCCCCC |
| Neural Network (NN) | CCCCCCC |
| Joint/Consensus | CCCCCCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
CancerPPD : Not available
ApIAPDB : Not available
CancerPPD2 ID: 7255
Reference
1 : Lin X, et al. Preparation and Characterization of an Anticancer Peptide from Oriental Tonic Food Enteromorpha prolifera. Foods. 2022; 11:(unknown pages). doi: 10.3390/foods11213507
Literature
Paper title : Preparation and Characterization of an Anticancer Peptide from Oriental Tonic Food Enteromorpha prolifera.
Doi : https://doi.org/10.3390/foods11213507
Abstract : Enteromorpha prolifera (E. prolifera), a tonic food in East Asian countries, is frequently studied for their pharmaceutical and healthcare applications. However, limited research has focused on antitumor peptides derived from this edible seaweed. In this study, we aimed to investigate the anticancer properties of peptides isolated from the hydrolysate of E. prolifera generated by a plethora of proteases including trypsin, papain, bromelain, and alkaline protease. The results showed that the hydrolysate produced by papain digestion exhibited remarkably stronger anticancer activity and was subjected to further purification by ultrafiltration and sequential chromatography. One heptapeptide, designated HTDT-6-2-3-2, showed significant antiproliferation activity towards several human cancer cell lines. The IC<sub>50</sub> values for NCI-H460, HepG2, and A549 were 0.3686 ± 0.0935 mg/mL, 1.2564 ± 0.0548 mg/mL, and 0.9867 ± 0.0857 mg/mL, respectively. Moreover, results from flow cytometry confirmed that cell apoptosis was induced by HTDT-6-2-3-2 in a dose-dependent manner. The amino acid sequence for this heptapeptide, GPLGAGP, was characterized by Edman degradation and further verified by Liquid Chromatography-Tandem Mass Spectrometry. In silico analysis results suggested that XIAP could be a potential target for HTDT-6-2-3-2. Molecular docking simulation showed that HTDT-6-2-3-2 could occupy a shallow pocket in the BIR3 domain of XIAP, which is involved in the inhibitory effect of caspase-9 activation. In conclusion, this E. prolifera derived peptide exhibited strong anticancer properties, which could be explored for pharmaceutical applications.