dbacp07276
General Description
Peptide name : LCP-2
Source/Organism : Laminaria japonica
Linear/Cyclic : Cyclic
Chirality : L
Sequence Information
Sequence : WAHT
Peptide length: 4
C-terminal modification: Cyclic
N-terminal modification : Cyclized
Non-natural peptide information:
Activity Information
Assay type : MTT assay
Assay time : 48-h
Activity : IC50 = 107 ± 11.8 μM
Cell line : MKN-45
Cancer type : Gastric Cancer
Other activity : Anticancer
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 513.5463 Dalton
Aliphatic index : 0.25
Instability index : -70.15
Hydrophobicity (GRAVY) : -0.75
Isoelectric point : 6.7411
Charge (pH 7) : -0.1527
Aromaticity : 25
Molar extinction coefficient (cysteine, cystine): (0, 0)
Hydrophobic/hydrophilic ratio : 1
hydrophobic moment : 0.3611
Missing amino acid : C,D,E,F,G,I,K,L,M,N,P,Q,R,S,V,Y
Most occurring amino acid : W
Most occurring amino acid frequency : 1
Least occurring amino acid : W
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (25, 0, 50)
SMILES Notation: C[C@H](NC(=O)[C@@H](N)Cc1c[nH]c2ccccc12)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@H](C(=O)O)[C@@H](C)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | HHHH |
| Chou-Fasman (CF) | CCCC |
| Neural Network (NN) | CCCC |
| Joint/Consensus | CCCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
CancerPPD : Not available
ApIAPDB : Not available
CancerPPD2 ID: 6739
Reference
1 : Du X, et al. Laminaria japonica cyclic peptides exert anti-colorectal carcinoma effects through apoptosis induction in vitro and in vivo. J Pept Sci. 2022; 28:e3385. doi: 10.1002/psc.3385
Literature
Paper title : Laminaria japonica cyclic peptides exert anti-colorectal carcinoma effects through apoptosis induction in vitro and in vivo.
Doi : https://doi.org/10.1002/psc.3385
Abstract : Laminaria japonica is consumed as a health food and used as a traditional medicine because of its biochemical and pharmacological abilities. However, the anti-tumor effect of L. japonica peptides has not been well explored. In the current study, three novel L. japonica cyclic peptides (LCPs) were isolated using an anti-cancer activity tracking method. LCP-3 [cyclo-(Trp-Leu-His-Val)] significantly induced apoptosis in Caco-2 cells in vitro. LCP-3 increased the Bax/Bcl-2 ratio, activated caspases, and regulated the p53/murine double minute 2 network. LCP-3 blocked Caco-2 cells in G0/G1 phase, which was accompanied by the inhibition of cyclin expression. Furthermore, LCP-3 inhibited colon cancer growth and induced cancer cell apoptosis in tumor-bearing mice. Notably, LCP-3 might be a potential agent for the prevention of colon cancer.