dbacp07285
General Description
Peptide name : AtMP2
Source/Organism : Anabastestudineus skin mucus fraction 2
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : TGIATSGLATFTLHTGSLAPAT
Peptide length: 22
C-terminal modification: Linear
N-terminal modification : Free
Non-natural peptide information:
Activity Information
Assay type : MTT assay
Assay time : 48-h
Activity : IC50 = 5.89 ± 0.14 μg/ml
Cell line : MCF-7
Cancer type : Breast Cancer
Other activity : Antimicrobial and Anticancer
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 2088.3175 Dalton
Aliphatic index : 0.890
Instability index : 17.6591
Hydrophobicity (GRAVY) : 0.6409
Isoelectric point : 6.4015
Charge (pH 7) : -0.5146
Aromaticity : 4.545
Molar extinction coefficient (cysteine, cystine): (0, 0)
Hydrophobic/hydrophilic ratio : 1.4444
hydrophobic moment : 0.0306
Missing amino acid : C,D,E,K,M,N,Q,R,V,W,Y
Most occurring amino acid : T
Most occurring amino acid frequency : 6
Least occurring amino acid : I
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (31., 27., 50.)
SMILES Notation: CC[C@H](C)[C@H](NC(=O)CNC(=O)[C@@H](N)[C@@H](C)O)C(=O)N[C@@H](C)C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@H](C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@H](C(=O)O)[C@@H](C)O)[C@@H](C)O)[C@@H](C)O)[C@@H](C)O)[C@@H](C)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | EEEEETTEEEEEEEETCCCCCC |
| Chou-Fasman (CF) | EEECCCCCEEEEECCCCCCCCC |
| Neural Network (NN) | CCCEECCCEEEEECCCCCCCCC |
| Joint/Consensus | EEEEECCCEEEEECCCCCCCCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
CancerPPD : Not available
ApIAPDB : Not available
CancerPPD2 ID: 6800
Reference
1 : Najm AAK, et al. Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions. Sci Rep. 2021; 11:23182. doi: 10.1038/s41598-021-02007-6
Literature
Paper title : Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions.
Doi : https://doi.org/10.1038/s41598-021-02007-6
Abstract : Previous study has shown the antimicrobial activities of mucus protein extracted from Anabas testudineus. In this study, we are interested in characterizing the anticancer activity of the A. testudineus antimicrobial peptides (AMPs). The mucus was extracted, fractioned, and subjected to antibacterial activity testing to confirm the fish's AMPs production. The cytotoxic activity of each fraction was also identified. Fraction 2 (F2), which shows toxicity against MCF7 and MDA-MB-231 were sent for peptide sequencing to identify the bioactive peptide. The two peptides were then synthetically produced and subjected to cytotoxic assay to prove their efficacy against cancer cell lines. The IC<sub>50</sub> for AtMP1 against MCF7 and MDA-MB-231 were 8.25 ± 0.14 μg/ml and 9.35 ± 0.25 μg/ml respectively, while for AtMP2 it is 5.89 ± 0.14 μg/ml and 6.97 ± 0.24 μg/ml respectively. AtMP1 and AtMP2 treatment for 48 h induced breast cancer cell cycle arrest and apoptosis by upregulating the p53, which lead to upregulate pro-apoptotic BAX gene and downregulate the anti-apoptotic BCL-2 gene, consequently, trigger the activation of the caspase-3. This interaction was supported by docking analysis (QuickDBD, HPEPDOCK, and ZDOCK) and immunoprecipitation. This study provided new prospects in the development of highly effective and selective cancer therapeutics based on antimicrobial peptides.