Peptide name : HMP-S7

Source/Organism : Human Milk

Linear/Cyclic : Linear

Chirality : L

Peptide length: 17

C-terminal modification: Linear

N-terminal modification : Free

Non-natural peptide information:

Assay type : Trypan blue assay

Assay time : 24-h

Activity : IC50 = 89 ± 24 µM

Cell line : Raji

Cancer type : Lymphoma Cancer

Other activity : Anticancer

Amino acid composition bar chart :

Molecular mass : 2001.3741 Dalton

Aliphatic index : 1.205

Instability index : 17.5588

Hydrophobicity (GRAVY) : 0.0529

Isoelectric point : 11.166

Charge (pH 7) : 2.4581

Aromaticity : 11.76

Molar extinction coefficient (cysteine, cystine): (0, 0)

Hydrophobic/hydrophilic ratio : 1.125

hydrophobic moment : -1.067

Missing amino acid : C,D,E,G,H,M,Q,V,Y

Most occurring amino acid : L

Most occurring amino acid frequency : 3

Least occurring amino acid : F

Least occurring amino acid frequency : 1

Secondary structure fraction (Helix, Turn, Sheet): (35., 29., 47.)

SMILES Notation: CC[C@H](C)[C@H](NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@@H](N)CO)[C@@H](C)CC)[C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)O

1 : Chiangjong W, et al. HMP-S7 Is a Novel Anti-Leukemic Peptide Discovered from Human Milk. Biomedicines. 2021; 9:(unknown pages). doi: 10.3390/biomedicines9080981

Paper title : HMP-S7 Is a Novel Anti-Leukemic Peptide Discovered from Human Milk.

Doi : https://doi.org/10.3390/biomedicines9080981

Abstract : Chemotherapy in childhood leukemia is associated with late morbidity in leukemic survivors, while certain patient subsets are relatively resistant to standard chemotherapy. It is therefore important to identify new agents with sensitivity and selectivity towards leukemic cells, while having less systemic toxicity. Peptide-based therapeutics has gained a great deal of attention during the last few years. Here, we used an integrative workflow combining mass spectrometric peptide library construction, in silico anticancer peptide screening, and in vitro leukemic cell studies to discover a novel anti-leukemic peptide having 3+ charges and an alpha helical structure, namely HMP-S7, from human breast milk. HMP-S7 showed cytotoxic activity against four distinct leukemic cell lines in a dose-dependent manner but had no effect on solid malignancies or representative normal cells. HMP-S7 induced leukemic cell death by penetrating the plasma membrane to enter the cytoplasm and cause the leakage of lactate dehydrogenase, thus acting in a membranolytic manner. Importantly, HMP-S7 exhibited anti-leukemic effects against patient-derived leukemic cells ex vivo. In conclusion, HMP-S7 is a selective anti-leukemic peptide with promise, which requires further validation in preclinical and clinical studies.