dbacp07430
General Description
Peptide name : GP-3
Source/Organism : Ginseng Leaf Peptide
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : FSHTYV
Peptide length: 6
C-terminal modification: Linear
N-terminal modification : Amidation
Non-natural peptide information:
Activity Information
Assay type : MTT assay
Assay time : 48-h
Activity : IC50 = 162 ± 17.1 µM
Cell line : CaCo-2
Cancer type : Colon Cancer
Other activity : Anticancer
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 752.8138 Dalton
Aliphatic index : 0.483
Instability index : -4.2333
Hydrophobicity (GRAVY) : 0.1667
Isoelectric point : 6.7402
Charge (pH 7) : -0.1537
Aromaticity : 33.33
Molar extinction coefficient (cysteine, cystine): (0, 0)
Hydrophobic/hydrophilic ratio : 0.5
hydrophobic moment : 0.2304
Missing amino acid : A,C,D,E,G,I,K,L,M,N,P,Q,R,W
Most occurring amino acid : F
Most occurring amino acid frequency : 1
Least occurring amino acid : F
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (0, 16., 66.)
SMILES Notation: CC(C)[C@H](NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)Cc1ccccc1)[C@@H](C)O)C(=O)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | EEEEEE |
| Chou-Fasman (CF) | EEECCC |
| Neural Network (NN) | CCCCEE |
| Joint/Consensus | EEEEEE |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
CancerPPD : Not available
ApIAPDB : Not available
CancerPPD2 ID: 6773
Reference
1 : Liu Z, et al. Anti-colon cancer activity tracking isolation of peptide from ginseng leaves and potential mechanisms evaluation in vitro and in vivo. J Pept Sci. 2021; 27:e3297. doi: 10.1002/psc.3297
Literature
Paper title : Anti-colon cancer activity tracking isolation of peptide from ginseng leaves and potential mechanisms evaluation in vitro and in vivo.
Doi : https://doi.org/10.1002/psc.3297
Abstract : The ginseng has been used for over hundred years, in the belief of promoting longevity. However, the anticancer activity of ginseng leaf peptide (GP) has been never explored. In current study, we isolated the GPs and explored the anti-colon cancer activity in vitro and in vivo. MTT results showed that the GP-1 (GP-1~FKEHGY) performed most antiproliferative activity against colon cancer CT-26 cells with an IC<sub>50</sub> of 86.4 ± 9.46 μM (48 h). Further study indicated that GP-1 activated the caspases, regulated the p53/murine double minute 2 (MDM2) state, and induced the CT-26 cells apoptosis in a mitochondrial pathway. Meanwhile, the GP-1 arrested the CT-26 cells in G0/G1 phase accompanied with cyclin expression regulation. In addition, GP-1 significantly suppressed the tumor growth and induced the tumor cells apoptosis in vivo. Notably, the GP-1 would be a potential anti-colon cancer candidate.