dbacp07511
General Description
Peptide name : YT12
Source/Organism : Arthrospira platensis
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : YFYPKDFTPGCT
Peptide length: 12
C-terminal modification: Linear
N-terminal modification : Free
Non-natural peptide information:
Activity Information
Assay type : MTT assay
Assay time :
Activity : IC50 = 12.5 μM
Cell line : HT-29
Cancer type : Colon Cancer
Other activity : Anticancer
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 1438.6014 Dalton
Aliphatic index : 0
Instability index : 67.5008
Hydrophobicity (GRAVY) : -0.575
Isoelectric point : 5.8273
Charge (pH 7) : -0.2517
Aromaticity : 33.33
Molar extinction coefficient (cysteine, cystine): (1, 0)
Hydrophobic/hydrophilic ratio : 1
hydrophobic moment : -0.319
Missing amino acid : A,E,H,I,L,M,N,Q,R,S,V,W
Most occurring amino acid : Y
Most occurring amino acid frequency : 2
Least occurring amino acid : K
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (8.3, 33., 50.)
SMILES Notation: C[C@@H](O)[C@H](NC(=O)[C@H](CS)NC(=O)CNC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H]1CCCN1C(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@@H](N)Cc1ccc(O)cc1)[C@@H](C)O)C(=O)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | ECCCTTCCTTCC |
| Chou-Fasman (CF) | CCCCCCCCCCCC |
| Neural Network (NN) | CCCCCCCCCCCC |
| Joint/Consensus | CCCCCCCCCCCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
CancerPPD : Not available
ApIAPDB : Not available
CancerPPD2 ID: 6645
Reference
1 : Sannasimuthu A, et al. Peroxiredoxin of Arthrospira platensis derived short molecule YT12 influences antioxidant and anticancer activity. Cell Biol Int. 2020; 44:2231-2242. doi: 10.1002/cbin.11431
Literature
Paper title : Peroxiredoxin of Arthrospira platensis derived short molecule YT12 influences antioxidant and anticancer activity.
Doi : https://doi.org/10.1002/cbin.11431
Abstract : This study demonstrates both the antioxidant and anticancer potential of the novel short molecule YT12 derived from peroxiredoxin (Prx) of spirulina, Arthrospira platensis (Ap). ApPrx showed significant reduction in reactive oxygen species (ROS) against hydrogen peroxide (H<sub>2</sub> O<sub>2</sub> ) stress. The complementary DNA sequence of ApPrx contained 706 nucleotides and its coding region possessed 546 nucleotides between position 115 and 660. Real-time quantitative reverse transcription polymerase chain reaction analysis confirmed the messenger RNA expression of ApPrx due to H<sub>2</sub> O<sub>2</sub> exposure in spirulina cells at regular intervals, in which the highest expression was noticed on Day 20. Cytotoxicity assay was performed using human peripheral blood mononuclear cells, and revealed that at 10 μM, the YT12 did not exhibit any notable toxicity. Furthermore, ROS scavenging activity of YT12 was performed using DCF-DA assay, in which YT12 scavenged a significant amount of ROS at 25 μM in H<sub>2</sub> O<sub>2</sub> -treated blood leukocytes. The intracellular ROS in human colon adenocarcinoma cells (HT-29) was regulated by oxidative stress, where the YT12 scavenges ROS in HT-29 cells at 12.5 μM. Findings show that YT12 peptide has anticancer activity, when treated against HT-29 cells. Through the MTT assay, YT12 showed vital cytotoxicity against HT-29 cells. These finding suggested that YT12 is a potent antioxidant molecule which defends ROS against oxidative stress and plays a role in redox balance.