dbacp07878
General Description
Peptide name : Fi-Histin1-21
Source/Organism : Fenneropenaeus indicus
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : SRSSRAGLQFPVGRIHRLLRK
Peptide length: 21
C-terminal modification: Linear
N-terminal modification : Amidation
Non-natural peptide information:
Activity Information
Assay type : XTT assay
Assay time : 24-h
Activity : IC50 = 31.274 ± 24.531 μM
Cell line : Hep-2
Cancer type : Liver Cancer
Other activity : Antimicrobial
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 2434.8474 Dalton
Aliphatic index : 0.928
Instability index : 99.2429
Hydrophobicity (GRAVY) : -0.628
Isoelectric point : 12
Charge (pH 7) : 5.5463
Aromaticity : 4.761
Molar extinction coefficient (cysteine, cystine): (0, 0)
Hydrophobic/hydrophilic ratio : 0.9091
hydrophobic moment : 0.8023
Missing amino acid : C,D,E,M,N,T,W,Y
Most occurring amino acid : R
Most occurring amino acid frequency : 5
Least occurring amino acid : A
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (23., 28., 28.)
SMILES Notation: CC[C@H](C)[C@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H](N)CO)C(C)C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCCN)C(=O)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | ETTTTTTEECEEHHHHHHHHH |
| Chou-Fasman (CF) | CCCCCCCCEEEEEEECCCCCC |
| Neural Network (NN) | CCCCCCCCCCCCCHHHHHHHH |
| Joint/Consensus | CCCCCCCCEEEECHHHHHHHH |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
CancerPPD : Not available
ApIAPDB : Not available
CancerPPD2 ID: 6198
Reference
1 : Sruthy KS, et al. A histone H2A derived antimicrobial peptide, Fi-Histin from the Indian White shrimp, Fenneropenaeus indicus: Molecular and functional characterization. Fish Shellfish Immunol. 2019; 92:667-679. doi: 10.1016/j.fsi.2019.06.044
Literature
Paper title : A histone H2A derived antimicrobial peptide, Fi-Histin from the Indian White shrimp, Fenneropenaeus indicus: Molecular and functional characterization.
Doi : https://doi.org/10.1016/j.fsi.2019.06.044
Abstract : Antimicrobial peptides (AMPs) derived from histone proteins form an important category of peptide antibiotics. Present study deals with the molecular and functional characterization of a 27-amino acid histone H2A derived AMP from the Indian White shrimp, Fenneropenaeus indicus designated as Fi-Histin. This peptide displayed distinctive features of AMPs such as amphiphilic alpha helical structure and a net charge of +6. The synthetic peptide exhibited significant antimicrobial activity against Gram-negative and Gram-positive bacteria especially against V. vulnificus, P. aeruginosa, V. parahaemolyticus, V. cholera and S. aureus. Disruption of cell membrane and cell content leakage were observed in peptide treated V. vulnificus using scanning electron microscopy. The synthetic peptide Fi-His<sub>1-21</sub> exhibited DNA binding activity and found to be non-haemolytic at the tested concentrations. Peptide was also found to possess anticancer activity against NCI-H460 and HEp-2 cell lines with an IC<sub>50</sub> of 22.670 ± 13.939 μM and 31.274 ± 24.531 μM respectively. This is the first report of a histone H2A derived peptide from F. indicus with a specific antimicrobial activity and anticancer activity, which could be a new candidate for future applications in aquaculture and medicine.