dbacp08264
General Description
Peptide name : HR
Source/Organism : Fraction identified from Tr1
Linear/Cyclic : Linear
Chirality : L
Sequence Information
Sequence : HVLSRAPR
Peptide length: 8
C-terminal modification: Linear
N-terminal modification : Free
Non-natural peptide information:
Activity Information
Assay type : MTT assay
Assay time : 48-h
Activity : 20% inhibition at 500 μg/mL
Cell line : SGC-7901
Cancer type : Gastric Cancer
Other activity : Anticancer
Physicochemical Properties
Amino acid composition bar chart :
Molecular mass : 935.085 Dalton
Aliphatic index : 0.975
Instability index : 47.475
Hydrophobicity (GRAVY) : -0.6
Isoelectric point : 12
Charge (pH 7) : 1.8473
Aromaticity : 0
Molar extinction coefficient (cysteine, cystine): (0, 0)
Hydrophobic/hydrophilic ratio : 1
hydrophobic moment : -2.019
Missing amino acid : C,D,E,F,G,I,K,M,N,Q,T,W,Y
Most occurring amino acid : R
Most occurring amino acid frequency : 2
Least occurring amino acid : H
Least occurring amino acid frequency : 1
Structural Information
3D structure :
Secondary structure fraction (Helix, Turn, Sheet): (25, 25, 25)
SMILES Notation: CC(C)C[C@H](NC(=O)[C@@H](NC(=O)[C@@H](N)Cc1c[nH]cn1)C(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCNC(=N)N)C(=O)O
Secondary Structure :
| Method | Prediction |
|---|---|
| GOR | EEEETCTT |
| Chou-Fasman (CF) | EECCCCCC |
| Neural Network (NN) | ECCCCCCC |
| Joint/Consensus | EECCCCCC |
Molecular Descriptors and ADMET Properties
Molecular Descriptors: Click here to download
ADMET Properties: Click here to download
Cross Referencing databases
CancerPPD : Not available
ApIAPDB : Not available
CancerPPD2 ID: 5742
Reference
1 : Wang Z and Zhang X. Isolation and identification of anti-proliferative peptides from Spirulina platensis using three-step hydrolysis. J Sci Food Agric. 2017; 97:918-922. doi: 10.1002/jsfa.7815
Literature
Paper title : Isolation and identification of anti-proliferative peptides from Spirulina platensis using three-step hydrolysis.
Doi : https://doi.org/10.1002/jsfa.7815
Abstract : BACKGROUND: Spirulina platensis is an excellent source of proteins (>60%) that can be hydrolyzed into bioactive peptides. RESULTS: In this study, whole proteins of Spirulina platensis were extracted and hydrolyzed using three gastrointestinal endopeptidases (pepsin, trypsin and chymotrypsin). Subsequently, gel filtration chromatography was employed to separate hydrolysates, and four fractions (Tr1-Tr4) were obtained. Among them, Tr2 showed the strongest anti-proliferation activities on three cancer cells (MCF-7, HepG-2 and SGC-7901), with IC<sub>50</sub> values of <31.25, 36.42 and 48.25 µg mL-1 , respectively. Furthermore, a new peptide, HVLSRAPR, was identified from fraction Tr1. This peptide exhibited strong inhibition on HT-29 cancer cells with an IC<sub>50</sub> value of 99.88 µg mL-1 . CONCLUSION: Taken together, these peptides possessed anti-proliferation activities on cancer cells and low cytotoxicity on normal cells, suggesting that they might serve as a natural anticancer agent for nutraceutical and pharmaceutical industries. © 2016 Society of Chemical Industry.